摘要
微生物菌株RS1是从江西鹰潭油茶酸性土壤中筛选获得的一株高耐铝红酵母,能够忍耐高达200 mmol/L以上的铝浓度。前期研究表明RS1可以把铝固定在细胞表面,阻挡其进入细胞内部,但是细胞表面何种基团参与铝的固定并不清楚。本文综合采用傅里叶红外光谱和拉曼光谱技术研究了铝胁迫下红酵母RS1细胞表面官能团变化,以期从光谱学角度来探索RS1的高耐铝机制。研究发现,70 mmol/L铝处理24 h后RS1红外光谱中1403 cm–1处的吸收峰红移到1397 cm–1处,这可能是羧基峰;在1706 cm–1处出现新的吸收峰,这可能是羰基峰;酰胺I带吸收峰强度显著上升,且甘露聚糖的吸收峰消失。拉曼光谱在779、856 cm–1处和1270 cm–1处出现新的吸收峰,可能分别为核糖核酸、酪氨酸和酰胺Ш带的吸收峰。综合结果表明,RS1细胞表面与铝吸附相关的物质主要是细胞壁多糖和蛋白质,主要涉及的官能团包括羧基、羰基和酰胺基。这些细胞表面官能团对铝的固定作用可能是红酵母RS1高耐铝的一个重要机制。
RS1 is a high aluminum resistant Rhodotorula taiwanensis strain selected from acid soil of oil tea in Yingtan of Jiangxi Province,which can tolerate aluminum concentration up to 200 mmol/L.Previous studies have showed that RS1 can immobilize aluminum on the cell surface and block it from entering the cell.However,it is unclear what kind of group on the cell surface is involved in aluminum fixation.In this paper,Fourier transform infrared spectroscopy and Raman spectroscopy were used to study the surface functional group changes of RS1 cells under aluminum stress in order to explore the high aluminum resistance mechanism of RS1 from the perspective of spectroscopy.It was found that the absorption peak at 1403 cm–1 in the IR spectrum of RS1 was red shifted to 1397 cm–1 after treatment with 70 mmol/L aluminum for 24 h,which may be a carboxyl group.A new absorption peak appeared at 1706 cm–1,which may be a carbonyl peak.The absorption peak intensities of amide I band in the IR spectrum with aluminum treatment were significantly increased and the mannan peak disappeared.The new absorption peaks of the Raman spectrum at 779 cm–1,856 cm–1 and 1270 cm–1 may be the absorption peaks of RNA,tyrosine and amideШband,respectively.The results of IR and Raman spectroscopy show that the substances related to aluminum adsorption on the surface of RS1 cells are mainly polysaccharides and proteins of cell wall,and the main functional groups include carboxyl groups,carbonyl groups and amide groups.The immobilization of aluminum by these functional groups on cell surface may be an important mechanism for the high aluminum tolerance of Rhodotorula taiwanensis RS1.
作者
程思
王超
沈仁芳
CHENG Si;WANG Chao;SHEN Renfang(State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《土壤》
CAS
CSCD
北大核心
2020年第6期1158-1163,共6页
Soils
基金
国家自然科学基金项目(41501328)
中国科学院战略性先导科技专项重点研究计划项目(XDB15030302)资助。
关键词
红酵母
铝毒
傅里叶红外光谱
拉曼光谱
Rhodotorula
Aluminum toxicity
Fourier transform infrared spectrum
Raman spectroscopy
