摘要
对丙酮处理酵母菌吸附铅前后的红外光谱进行了分析比较。生物材料的红外光谱图主要由蛋白质的吸收带、碳水化合物的吸收带组成。1652cm-1、1534cm-1、1239cm-1处的吸收峰是蛋白质的三个特征吸收峰。1455cm-1处的吸收峰为CH3和CH2的弯曲振动峰;1397cm-1处中等强度的吸收归属于羧基的对称伸缩振动。1200～940cm-1之间出现的强且复杂的谱带归属于醚和碳水化合物中的C-O伸缩振动。915cm-1、890cm-1处两个弱的吸收峰属于细胞壁中多糖的糖环键的振动。吸附Pb2+后,3375cm-1处的吸收峰紫移至3388cm-1,1398cm-1处的峰红移到1384cm-1,说明羟基和羧基在结合Pb2+起重要作用。丙酮处理酵母菌的主要成份和结构吸附前后保持完整。
The acetone-washed yeast and lead-exposed yeast biomass were examined by infrared spectroscopy. The IR spectroscopy of biomass is mainly composed by the adsorption of carbohydrates, protein, etc. The dominating bands near 1652, 1534 and 1239cm^(-1) are assigned to amide I, amide II and amide Ⅲ, the characteristic IR absorption of protein which could be one of the significant components of cell walls. The peak 1455 cm^(-1) is attributable to the bending stretching of CH_(2^-) and CH_(3^-). Strong and complex bands attributed to ether and hydroxyl C-O stretching between 1200 and 940cm^(-1). Two weak absorption peaks at 915 and 890cm^(-1) can be attributed to the glycoside bonds in the polysaccharides structure of the acetone-washed biomass. The changes in the spectrum of the lead-exposed yeast biomass were in the stretch of hydroxyl groups, which shifted from 3374 to 3388cm^(-1) and in the symmetrical stretch of the carboxylate groups, which shifted from 1398 to 1384cm^(-1). The components and structure of the exposed-lead biomass remain intact.
出处
《河南科学》
2004年第6期777-779,共3页
Henan Science
基金
河南省科技攻关资助项目(012409041)
