摘要
以枇杷、茄子和水杉等45种植物的健康叶片或茎中分离得到的80株植物内生菌为试材,采用平板对峙法,研究了这些菌株对病原菌树生黄单胞菌核桃致病变种Xanthomonas arboricola pv.juglandis的影响,以期为核桃细菌性疫病的生物防治提供理论参考依据。结果表明:得到5株有明显拮抗作用的菌株,分别是PPE17-18、QCE17-2、QZYE-41、QZJ-42和SSE17-24。其中菌株QZYE-41抑菌能力最强,PPE17-18和SSE17-24的抑菌能力较弱,5株菌株均为革兰氏阳性菌。通过16S rDNA和gyrB基因测序分析,结合菌落形态和生理生化测试,初步确定QZYE-41和SSE17-24为枯草芽孢杆菌(Bacillus subtilis),PPE17-18和QZJ-42为鲍曼不动杆菌(Acinetobacter baumannii),QCE17-2为不动杆菌属(Acinetobacter sp.)。该研究为核桃细菌性疫病的生物防治菌的开发应用提供了潜在生物新材料。
In this study,including loquat,eggplant and metasequoia,45 kinds plants healthy leaves or stems were used as experiment materials,80 strains of endophytic bacteria were isolated.By using plate-confrontation assay,the interactions were studied between the isolated strains and the walnut pathogenic bacterium Xanthomonas arboricola pv.juglandis,respectively,in order to provide theoretical basis for the biological control of walnut bacterial blight.The results showed that five of the isolated,PPE17-18,QCE17-2,QZYE-41,QZJ-42 and SSE17-24,had obvious antagonistic effects.Among them,QZYE-41 had the strongest antibacterial ability,while PPE17-18 and SSE17-24 had weaker antibacterial ability.All the five strains were gram-positive bacteria.Through 16 SrDNA and gyrB gene sequencing analysis,combined with colony morphology and physiological and biochemical tests,it was preliminarily determined that both QZYE-41 and SSE17-24 were Bacillus subtilis,PPE17-18 and QZJ-42 were Acinetobacter baumannii,and QCE17-2 was Acinetobacter sp.This study provided a potential new material for the developing and application for biological control of walnut bacterial blight.
作者
涂昌
柯文山
邹路路
张程
傅本重
王立华
TU Chang;KE Wenshan;ZOU Lulu;ZHANG Cheng;FU Benzhong;WANG Lihua(Hubei Key Laboratory of Quality and Safety Control of Characteristic Fruits and Vegetables,Hubei Engineering University,Xiaogan,Hubei 432000;College of Life Sciences,Hubei University,Wuhan,Hubei 430062)
出处
《北方园艺》
CAS
北大核心
2020年第23期10-16,共7页
Northern Horticulture
基金
2019年中央引导地方科技发展专项资助项目(2019ZYYD045)。
关键词
内生菌
核桃细菌性疫病
拮抗作用
筛选
鉴定
endophytic bacteria
bacterial blight of walnut
antagonism
screening
identification
