摘要
在对DNA条形码(DNA barcode)技术发展进程分析归纳的基础上,对已发表的各门藻类DNA条形码序列进行总结。现阶段较为常用的DNA条形码基因主要包括ITS、cox1、rbcL等基因,几种常用基因片段各自存在优缺点,本文对各片段的主要问题进行了分析。其中原核蓝藻的ITS基因和藻胆蛋白基因可考虑作为首选DNA条形码,真核藻类主要考虑ITS和rbcL基因,很难找到像动物cox1基因可在物种鉴定中广泛通用的DNA条形码。不同基因片段间互补应用,并与传统形态学鉴定方法相结合可有效提高近缘藻类分类的准确性。从藻类DNA条形码研究的新进展及其应用前景来看,藻类DNA条形码的研究重点仍是加快开发新的DNA条形码片段并对其进行评价,为寻找理想的通用DNA条形码提供理论支持。
Barcodes for the DNA sequences of algal genes have gradually come into use because they provide aquick,effective means for identifying algae in biodiversity studies.In this study,we summarized com-monly used barcodes of algae genes and,based on an analysis of DNA barcode technology,identified their advantages and disadvantages.At present,the most commonly barcoded genes include ITS,cox 1,rbc L.Barcodes of DNA sequences in the ITS and phycobiliprotein genes is now the preferred method of identif-ying prokaryotic cyanobacteria,and barcodes of the ITS and rbc L genes have been found suitable for eu-karyotic algae.However,it should be noted that finding a suitable gene for DNA barcoding in animals,that can be widely used for species identification,has proven difficult.Therefore,current research on DNA barcoding needs to focus on accelerating the development of new DNA barcode fragments for algae and e-valuating their utility.This will provide theoretical support in the search for ideal universal DNA barcodes.Furthermore,traditional morphological identification methods,complemented by gene fragment identifica-tion,will improve accuracy when classifying closely related algae species.
作者
毛启迪
杨苏文
金位栋
闫振广
闫玉红
焦立新
徐彬
焦巨龙
MAO Qi-di;YANG Su-wen;JIN Wei-dong;YAN Zhen-guang;YAN Yu-hong;JIAO Li-xin;XU Bin;JIAO Ju-long(State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,P.R.China;National Engineering Laboratory for Lake Pollution Control and Ecological Restoration,Chinese Research Academy of Environmental Sciences,Beijing 100012,P.R.China;School of Resources Environmental and Chemical Engineering,Nanchang University,Nanchang 330031,P.R.China)
出处
《水生态学杂志》
CSCD
北大核心
2020年第6期9-18,共10页
Journal of Hydroecology
基金
中央级公益性科研院所基本科研业务专项(2016YSKY-006)。
关键词
藻类
DNA条形码
物种分类
应用研究
algae
DNA barcodes
species classification
application research
