摘要
目的探讨头孢吡肟(FEP)分别与美罗培南(MEM)和环丙沙星(CIP)联合应用对多重耐药铜绿假单胞菌(MDRPA)的体外抑菌作用的影响,为临床治疗MDRPA提供依据。方法采用棋盘法设计,通过琼脂平板稀释法测定抗菌药物对58株临床分离的MDRPA的最低抑菌浓度(MIC),通过计算分级抑菌浓度指数评价药物体外联合抑菌效果。结果FEP与MEM联合后,表现为协同作用的为32.8%,相加作用的为46.6%,无关作用的为20.6%,无拮抗作用;FEP与CIP联用后,表现为协同作用的为20.7%,相加作用的为51.7%,无关作用的为27.6%,无拮抗作用。联合应用的药物组合,各药MIC 50均明显降低,浓度-累积抑菌率曲线均表现为左移。结论FEP分别与MEM和CIP联用均可以提高各自对MDRPA抗菌作用的敏感性。
Objective To evaluate the combined inhibitory effect of cefepime(FEP)and meropenem(MEM),FEP and ciprofloxacin(CIP)respectively on clinically isolated multidrug resistant pseudomonas aeruginosa(MDRPA)strains in vitro.Methods Combined inhibitory effect of FEP and MEM,FEP and CIP in vitro was assayed by detecting the minimum inhibitory concentration(MIC)and calculating the fractional inhibitory concentration index for 58 MDRPA strains with the agar dilution method.Results Combined FEP and MEM showed 32.8%synergic effect,46.6%additive effect,20.6%unrelated effect,no antagonistic effect on MDRPA strains.Combined FEP and CIP displayed 20.7%synergic effect,51.7%additive effect,27.6%unrelated effect and no antagonistic effect on MDRPA strains.The MIC 50 of these drugs was significantly lower when they were used in combination,with their concentration-accumulative inhibition curves shifted to the left.Conclusion FEP combined with either MEM or CIP,compared with respective use,showes an elevated drug sensitivity for MDRPA strains in vitro.
作者
龚美亮
邹琳
刘云霞
GONG Mei-Liang;ZOU Lin;LIU Yun-Xia(Clinical Laboratory,Second Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Department of Health Care,Second Medical Center,Chinese PLA General Hospital,Beijing 100853,China)
出处
《中华老年多器官疾病杂志》
2020年第12期891-894,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
基金
军队后勤科研项目(CWS12J131)。
