摘要
利用生物基因组学数据库,对绵羊神经原相关的细胞粘附分子基因(neuro-related celladhesion molecule,NRCAM)进行生物信息学分析,以初步了解其结构并对其编码的蛋白质的功能进行预测。结果表明,绵羊NRCAM基因含有1个最大长度为3648 bp的开放阅读框,编码1215个氨基酸残基。NRCAM基因编码产物的分子质量为134367.13 KDa,理论等电点为5.49。亚细胞定位主要位于细胞质(26.1%),属于分泌蛋白,且存在信号肽序列。存在5段IGc2区、1段IG区,1段低复杂性区域以及4段FN3区,且有1段跨膜结构;二级结构以无规卷曲和β折叠为主,三级结构主要由无规卷曲和β折叠缠绕形成。
The bioinformatics analysis of sheep NRCAM gene was carried out by using bioinformatics database and software,and its structure was preliminarily understood and predicted.The results showed that sheep NRCAM gene contained an open reading frame of 3648 bp,encoding 1215 amino acid residues.The molecular mass of NRCAM protein was 134367.13 KDa,and the theoretical isoelectric point PI was 5.49.The subcells were mainly located in cytoplasmic(26.1%)and belong to secretory protein.There are signal peptide sequences.There are five segments of IGc2 regions,a segments of IG region,a segments of low complexity region,four segments of FN3 region and a segments of transmembrane structure.The secondary structure is mainly random crimp andβ-pleated sheet,and the tertiary structure is mainly formed by random crimp andβ-pleated sheet,winding and folding.
作者
靳泽希
冯芬
邓晓银
王维民
JIN Zexi;FENG Fen;DENG Xiaoyin;WANG Weimin(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou Gansu 730070,China;Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province,Minqin Gansu 733300,China)
出处
《甘肃农业科技》
2020年第12期19-24,共6页
Gansu Agricultural Science and Technology
基金
甘肃农业大学学生科研训练计划项目(202004013)。
