摘要
采用测序的方法鉴定136头中国荷斯坦奶牛ELOVL5基因启动子区域的遗传多态性位点,通过统计学分析遗传多态性位点与乳质性状的关联性,并根据差异显著性多态性位点构建ELOVL5基因启动子双荧光素酶报告载体,检测不同基因型的启动子活性。结果表明:在奶牛ELOVL5基因启动子区域中发现了7个SNPs及1个单碱基缺失突变,其中SNP7g.-385C>G与尿素氮、校正奶量显著相关(P<0.05),单碱基缺失突变与牛奶中的体细胞数极显著相关(P<0.01)。双荧光素酶报告载体检测结果显示,缺失纯合基因型的启动子活性极显著高于野生型(P<0.01)。
The objective of this study was to evaluate the association between ELOVL5 gene poly- morphisms and milk quality of Holstein cattle. Using direct DNA sequencing,seven single nucleo- tide polyraorphisrns（SNPs）and 1-bp del polymorphism were identified within the promoter region of ELOVL5 gene from 136 individuals. According to the statistical analysis,l-bp ins/del polymor- phism was significantly associated with somatic cell count（SCC）（P〈0.01） ,and SNPTg. -385C〉 G was significantly associated with urea nitrogen and corrected milk yield（P〈0.05）. Then the transcriptional activities of different genotypes were determined using a dual-luciferase reporter assay system in bovine mammary epithelial cells（MECs）,the homozygous 1-bp del mutation geno-type increased by a 1.59-fold（P〈0.01） activity of luciferase compared with the wild type.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第4期741-745,共5页
Chinese Journal of Veterinary Science
基金
国家科技重大专项资助项目(2016ZX08009003-006-002)
国家"863"计划资助项目(2013AA102505)
国家自然科学基金资助项目(31372278)
