摘要
以92份割手密初级核心种质为研究对象,利用SSR分子标记数据,依据Jaccard、SM和Nei&Li 3种遗传相似性系数逐步进行UPGMA聚类、多次抽样构建割手密核心种质库。结果显示:92份样本在15个SSR位点上呈现出丰富的多态性,共获得331个多态性条带,平均多态性条带比例达100%;利用3种遗传相似性系数和随机取样共获得5个核心种质库;Shannon–Wiener多样性指数、总条带数和多态性条带数3个指标在核心种质库代表性检验中呈现出较高的检测效率,其他6个指标检测效率相对较低;5个核心库中依据SM遗传相似性系数逐步构建的核心种质库质量最优,该库由80份样本组成,Nei’s多样性指数和Shannon−Wiener多样性指数分别为0.9841和4.2598,在P<0.05概率条件下与原库(分别为0.9856和4.3583)无显著差异,而且与原库的农艺性状均值差异百分率为0(<20.00%),极差符合率为99.16%(>80.00%)。研究认为,依据SM相似性系数,通过UPGMA聚类构建的80份割手密核心种质较好地代表了基础原始种质的分子水平和表型遗传多样性,可为后续割手密种质资源的精准鉴定、优异抗性基因发掘和种质资源杂交利用提供依据。
A stepwise UPGMA cluster sampling approach with three different genetic similarity coefficients(Jaccard,SM,and Nei&Li)was applied to a SSR molecular data of 92-genotype primary core collection.Results showed that 92 genotypes possessed abundant genetic diversity at 15 SSR loci,which produced 331 polymorphic bands in amplification,constituting a mean of 100%of total bands.The genetic similarity coefficients were constructed with three approaches and two random samplings,which produced five putative core collections.To evaluate the quality of putative core collections,three indices,Shannon-Wiener diversity index,polymorphic band number and percentage of polymorphic bands,using core collections had the highest identification efficiency,while others were less efficient.The putative core collection constructed using SM genetic similarity coefficient had higher quality than others.The putative core collection consisted of 80 Saccharum spontaneum genotypes shaving a 0.9841 Nei’s diversity index and 4.2598 Shannon-Wiener diversity index,and did not differ significantly(P<0.05)in molecular diversity from the primary core.Further,MD was 0%(<20.00%)and CR 99.16%(>80.00%).The putative core collection of 80 S.spontaneum genotypes based on SM genetic similarity coefficient did not differ significantly from the genetic diversity of the primary core regarding to the agronomic traits or molecular markers.The putative core will facilitate the evaluation and utilization of the germplasm in developing elite sugarcane hybrids,and mining elite genes.
作者
徐超华
刘新龙
毛钧
刘洪博
林秀琴
陆鑫
苏火生
XU Chaohua;LIU Xinlong;MAO Jun;LIU Hongbo;LIN Xiuqin;LU Xin;SU Huosheng(Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan 661699,China;Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan,Yunnan 661699,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第6期657-663,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
云南省农业基础研究联合专项(2018FG001-017)
国家农作物种质资源共享服务平台项目(NICGR-2019-44)
农业农村部政府购买服务项目(19190167)。
关键词
割手密
聚类
多样性指数
分子标记
核心种质
Saccharum spontaneum
clustering
diversity index
molecular marker
core collection
