摘要
目的从疾病和证候2个维度建立溃疡性结肠炎(ulcerative colitis,UC)大鼠脾虚湿蕴证辨识方法,为中药药效评价及证候基础研究提供适宜动物模型。方法将40只SD大鼠随机分为4周及8周的对照组与模型组,每组10只。采用免疫复合法造模,多时间点采集比较大鼠的宏观表征、抓力、毛发、旷场实验等方面数据,并分别于造模后28、56 d取材,HE染色分析结肠病理变化。将溃疡性结肠炎临床常见证候诊断标准等效转化,用于大鼠证候辨识。结果在造模后1~3 d,模型组大鼠不欲饮食,体重下降明显,腹泻便血,抓取反抗变强,但活跃度、反应能力、自主活动、精神状态均正常,毛发较暗淡凌乱。4 d^2周,模型组大鼠表现为稀便黏附肛门,宏观表征、抓力等与对照组基本无差异。在3~4周,模型组大鼠排稀便,饮食量及抓力降低(P<0.05),毛发暗淡凌乱,较对照组活跃度降低,抓取反抗及反应能力减弱,病理符合疾病诊断。5~8周,模型组各指标逐渐恢复正常。结论免疫复合法造模后3~4周模型动物符合溃疡性结肠炎脾虚湿蕴证。
Objective To investigate the differentiation methods for the pattern of spleen deficiency leading to damp accumulation in ulcerative colitis(UC) rats from the perspectives of both the disease in Western medicine and the pattern in traditional Chinese medicine(TCM), and then establish desirable models for pharmacological evaluation of Chinese herbal medicine and basic researches in TCM pattern.Methods 40 SD rats were randomly divided into the control groups(studied for 4 weeks) and the model groups(8 weeks), 10 in each group. Immune induction together with coloclysis of 2,4, 6-trinitrobenzene sulfonic acid(TNBS) was used to induce ulcerative colitis. Macroscopic characteristics, grip strength, open field test, hair and other features of the rats were collected at multiple time points. The colon samples were taken on the 28th and 56th days after coloclysis and the pathological changes were analyzed using HE stained sections. The corresponding criteria for pattern differentiation of rats were established based on common clinical diagnostic criteria of ulcerative colitis.Results Three days after coloclysis, rats in the model groups manifested relatively lusterless and frizzy hair, anorexia, significant weight loss, diarrhea, bloody stool, and increased resistance against grasping but normal vitality, responsiveness, autonomous activity and general state. From the fourth day to the end of the second week, compared with the control group, no significant differences were observed in the model rats except loose and sticky stool. In the third and fourth weeks, the model rats excreted loose stools, ate less and showed decreased grip strength(P<0.05) and lusterless and frizzy hair. Compared with the control groups, they also showed decreased vitality, resistance against grasping and responsiveness and UC was diagnosed based on their colonic pathological test results. From the fifth week to the eighth week, all the parameters of the model rats returned to normal gradually.Conclusion During the third and fourth weeks after coloclysis, the model rats could meet the diagnostic criteria of ulcerative colitis with spleen deficiency leading to damp accumulation pattern.
作者
焦文超
罗慧
唐家杨
王青
苏聪平
于雪
刘燕
石冰洁
王伟
郭淑贞
Jiao Wenchao;Luo Hui;Tang Jiayang;Wang Qing;Su Congping;Yu Xue;Liu Yan;Shi Bingjie;Wang Wei;Guo Shuzhen(School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2020年第9期738-745,共8页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家重点研发计划项目(No.2017YFC1700100,No.2017YFC1700105)。
关键词
溃疡性结肠炎
大鼠
证候评价
脾虚湿蕴证
ulcerative colitis
rat
pattern evaluation
spleen deficiency leading to damp accumulation pattern
