摘要
目的构建绝经后骨质疏松症(PMOP)大鼠模型,探讨白藜芦醇(RES)在PMOP中的促细胞增殖作用。方法将80只清洁级SD大鼠随机分为对照组、PMOP组、RES低剂量组(60 mg/kg)、RES中剂量组(80 mg/kg)、RES高剂量组(100 mg/kg),每组16只。大鼠行双侧卵巢摘除术,术后1周给予RES预防性治疗,每月称重体质量。24周后心脏采血处死,取双侧股骨,采用骨密度仪测量骨密度变化,通过逆转录聚合酶链式反应(RT-PCR)检测骨组织中p53和Cyclin-D1水平的变化,Western blot检测各组大鼠Notch-1蛋白表达情况。结果PMOP组大鼠股骨和椎骨的骨密度降低,RES不同剂量组大鼠股骨和椎骨的骨密度较PMOP组升高,差异均有统计学意义(P<0.05)。与对照组相比,PMOP组p53和Cyclin-D1的m RNA转录水平显著增高,RES不同剂量组的Cyclin-D1的m RNA转录水平较PMOP组升高,而p53的m RNA转录水平降低,差异均有统计学意义(P<0.05)。Western blot结果显示,PMOP组的Notch-1表达水平显著抑制,RES不同剂量组Notch-1蛋白表达较PMOP组升高,差异有统计学意义(P<0.05)。结论RES调控Notch-1信号通路来促进骨组织中的细胞增殖,发挥拮抗骨质疏松症的作用。
Objective To establish a rat model of postmenopausal osteoporosis(PMOP)and explore role of resveratrol(RES)in promoting cell proliferation of rats with PMOP.Methods Totally 80 clean SD rats were randomly divided into control group,PMOP group,low dose RES group(60 mg/kg),medium dose RES group(80 mg/kg)and high dose RES group(100 mg/kg),with 16 rats in each group.Rats were treated with bilateral ovariectomy and RES as preventive treatment one week after operation,and body weight was measured every month.After 24 weeks,blood samples were collected from the heart after sacrifice and bilateral femurs were taken.Bone mineral density was measured by bone densitometer,levels of p53 and Cyclin-D1 were detected by reverse transcription polymerase chain reaction(RT-PCR),and the expression of Notch-1 protein was detected by Western blot.Results The bone mineral densities of femur and vertebrae in the PMOP group decreased significantly,and bone mineral densities of femur and vertebrae in the groups with different RES concentrations were significantly higher than those in the PMOP group(P<0.05).Compared with the control group,the m RNA transcription levels of p53 and Cyclin-D1 in the PMOP group were significantly higher,and the m RNA transcription levels of Cyclin-D1 in the groups with different RES concentrations were significantly higher than that in the PMOP group,while the m RNA transcription level of p53 was significantly lower(P<0.05).Western blot results showed that the expression level of Notch-1 in the PMOP group was significantly inhibited,and the expression level of Notch-1 in the groups with different RES concentrations was significantly higher than that in the PMOP group(P<0.05).Conclusion RES plays a role of antagonizing osteoporosis through regulating Notch-1 signaling pathway for promotion of cell proliferation in bone tissue.
作者
邹庆峰
李守民
舒隆钧
刘世昌
张继源
ZOU Qingfeng;LI Shoumin;SHU Longjun;LIU Shichang;ZHANG Jiyuan(Department of Orthopedics,Dali First People’s Hospital in Yunnan Province,Dali,Yunnan,671000)
出处
《实用临床医药杂志》
CAS
2020年第19期86-89,共4页
Journal of Clinical Medicine in Practice
基金
云南省科技厅-昆明医科大学联合基金资助项目(2014FB006)
云南省大理市科技局基金资助项目(2016KJ35)。
