摘要
目的通过建立大鼠颈外静脉导管相关性血栓(CRT)模型,观察大鼠CRT的形成情况,同时检测大鼠血清中组织因子(TF)、血栓调节蛋白(TM)及血管性假血友病因子(vWF)的含量,研究其与CRT形成的关系。方法取雄性SD大鼠120只,随机分为空白对照组(n=40)、假手术组(n=40)、模型组(n=40),各组分别于术后1 d、4 d、7 d、10 d、14 d随机取大鼠8只,病理切片观察大鼠血栓形成情况,以ELISA法检测血清TF、TM、vWF含量。结果空白对照组及假手术组均无血栓形成,模型组有34只发生CRT,CRT发生率为85%。模型组发生CRT大鼠的TM含量较未发生CRT大鼠明显升高(P<0.01),模型组未发生CRT大鼠的TM含量较假手术组显著升高(P<0.01),模型组未发生CRT大鼠的TM、vWF含量较空白对照组明显升高(P<0.01)。术后各个时间点,模型组TF、TM含量均较空白对照组及假手术组明显升高(P<0.01)。术后4 d、7 d、10 d、14 d,模型组vWF含量较空白对照组及假手术组显著升高(P<0.01)。模型组术后随着观察时间的延长,大鼠血清TF、TM、vWF含量随时间逐渐增加,其中TF、vWF含量在术后1~4 d急剧上升,术后10 d达到峰值,随后急剧下降;TM含量在术后4~7 d增幅最大,术后7 d达到峰值,随后开始下降。结论大鼠CRT形成与血清中TF、TM及vWF的异常表达密切相关。
Objective To establish a rat model of external jugular vein catheter-related thrombosis(CRT),observe the formation of CRT in rats,detect the levels of tissue factor(TF),thrombomodulin(TM),and von Willebrand factor(vWF)in the serum of rats,and study their relationship with CRT formation.Methods A total of 120 male Sprague-Dawley rats were randomly divided into blank control(n=40),sham operation(n=40),and model(n=40)groups.Eight rats were randomly selected at 1,4,7,10,and 14 days and pathological sections were used to observe the thrombosis in the rats and serum TF,TM,and vWF levels were measured by enzyme linked immunosorbent assay.Results There was no thrombosis in blank control or sham operation groups.There were 34 CRTs in the model group,and the CRT incidence rate was 85%.The TM level in the model group with CRT rats was significantly higher than that without CRT rats(P<0.01).The TM level in the model group without CRT rats was significantly higher than that in the sham operation group(P<0.01).TM and vWF levels in rats without CRT were significantly higher than those in the blank control group(P<0.01).At each time point after surgery,TF and TM levels of the model group were significantly higher than those of blank control and sham operation groups(P<0.01).At 4,7,10,and 14 days after the operation,the vWF level of the model group was significantly higher than that in blank control and sham operation groups(P<0.01).Over a prolonged observation time in the model group,TF,TM,and vWF levels increased gradually,and the levels of TF and vWF increased sharply from 1 to 4 days after operation,which peaked at 10 days after the operation and then decreased rapidly.TM levels increased the most at 4-7 days after the operation,which peaked at 7 days after the operation and then began to decrease.Conclusions The formation of rat CRT is closely related to abnormal levels of TF,TM,and vWF in serum.
作者
韦艳
应燕萍
凌瑛
赵慧函
蒋庆娟
甘晓
文萃
WEI Yan;YING Yanping;LING Ying;ZHAO Huihan;JIANG Qingjuan;GAN Xiao;WEN Cui(the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第10期8-13,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(81860032)
广西自然科学基金项目(2018GXNSFAA050081)
广西研究生教育创新计划项目(YCSW2019115)。
