摘要
目的探讨蒿甲醚对小鼠恶唑酮结肠炎的抗炎作用及其机制。方法随机将40只BALB/c小鼠分为4组:空白对照组、结肠炎组、地塞米松磷酸钠(DX)治疗组和蒿甲醚治疗组,每组各10只。结肠炎模型制备先以恶唑酮皮肤涂抹致敏,6 d后再予恶唑酮灌肠。DX、蒿甲醚橄榄油溶液臀大肌注射治疗。小鼠造模4 d后采集血液、结肠等标本进行病理组织学和超微结构观察,作疾病活动指数(DAI)、普通病理及超微结构等方面的评定。TNF-α、IL-13等指标的测定采用ELISA方法。结果恶唑酮灌肠后小鼠出现稀便、大便隐血阳性及体质量下降等。结肠炎组、DX组和蒿甲醚组在灌肠造模后DAI评分均明显高于空白对照组。光镜下显示:结肠炎组小鼠肠黏膜局灶性隐窝增宽、变形,隐窝细胞和杯状细胞缺失,浅溃疡形成,大量炎症细胞浸润。电镜下显示:蒿甲醚组、DX组与结肠炎组相比,细胞结构较完整、炎症细胞较少、细胞器结构功能较正常。ELISA检测显示:蒿甲醚组和DX组的TNF-α表达低于结肠炎组,而IL-13表达高于结肠炎组。结论蒿甲醚给药后小鼠结肠炎病理损伤显著减轻,疗效与DX给药相近,某些指标可能优于后者;免疫学检测表明,蒿甲醚对小鼠结肠炎产生抗炎作用的同时呈现出TNF-α表达水平降低、IL-13表达水平增高的趋势。
Objective To investigate the anti-inflammatory action of Artemether on oxazolone colitis mice and its mechanism.Methods Forty BALB/c mice were devided into 4 groups,including control group,colitis group,dexamethasone sodium phosphate(DX)group and Artemether group.Skin application with Oxazolone for hyper-sensitization began 6 days before induction of colitis via enema.And DX,Artemether-olive oil were administered intramuscularly after another 4 days.Blood and colon samples were collected for microscopic detection and observation with transmission electron microscope along with ELISA for serum TNF-αand IL-13.And disease activity index(DAI)was used on the criteria by Cooper.Results The BALB/c mice had diarrhea,fecal occult blood positive,bodyweight loss after enema.Score of DAI in the 3 colitis groups were significantly higher than that in the control group.Swollen and distorted crypts with loss of goblet and crypt cells,superficial ulcers and infiltration of large amount of pre-inflammatory cells were found microscopically.And the ultra-microscopic findings of the injuries including infiltration of inflammatory cells,damaged cellular contour or twisted intracellular organelles were significantly alleviated as well.And finally,serum TNF-αlevel was lower in Artemether and DX group than that in colitis group,while IL-13,significantly higher in Artemether and DX group than that in colitis group.Conclusion Score of mucosal injury in Artemether group,similar to DX group with some better parameters,are significantly decreased compared with that in colitis group;And TNF-αis declined while IL-13 significantly elevated in Artemether group,which may be attributed to the anti-inflammatory protection.
作者
沈天天
SHEN Tiantian(Department of Internal Medicine,Shanghai Dianli Hospital,Shanghai 200050,China)
出处
《胃肠病学和肝病学杂志》
CAS
2020年第10期1156-1161,共6页
Chinese Journal of Gastroenterology and Hepatology
关键词
蒿甲醚
炎症性肠病
结肠炎
小鼠
细胞因子
Artemether
Inflammatory bowel disease
Colitis
Mice
Cytokines
