摘要
为了从分子水平分析山西省高粱地方品种的遗传多样性和遗传结构,本研究从140对SSR引物中筛选到22对条带清晰且多态性稳定的引物,对来自于山西省11个地区66个县市的158份高粱材料进行了遗传多样性分析,结果共检测到147个等位基因的位点变异,平均等位基因数为6.68。每个SSR位点的多态性信息含量(PIC)平均为0.635,变异范围为0.168~0.870,表明山西高粱的地方品种具有较丰富的多态性。针对11个不同地理居群的遗传多样性分析表明,不同的地理居群间遗传分化水平差异较大,地理居群间存在不同程度的基因交流。无论从等位基因数、有效等位基因数还是Shannon's信息指数上,都可以看出忻州居群的遗传多样性最高,而阳泉居群的最低。针对不同地理居群间遗传距离的分析结果表明,忻州居群和晋中居群的遗传距离最小,两个群体的亲缘关系较近,基因交流比较频繁。而阳泉居群和其他居群间的遗传距离都较大,显示阳泉居群与其他居群之间的基因交流阻隔,进一步显示了阳泉居群的独特性。基于模型的遗传结构分析和基于遗传距离的N-J聚类分析都将种质材料划分为2个类群,不同地区高粱资源居群间的遗传关系远近与其地理信息并没有明显的相关性。本研究旨在为高粱种质资源的收集、鉴定与创新提供理论依据。
In order to analyze the genetic diversity and structure of sorghum local cultivars in Shanxi Province from the molecular level,22 pairs of SSR primers with clear and stable polymorphisms were screened from 140 pairs of SSR primers,and used to analyze 158 sorghum materials from 66 counties and cities in 11 regions of Shanxi Province.The results showed that a total of 147 alleles loci were detected with an average number of alleles 6.68.The polymorphism information content(PIC)of each SSR locus averaged 0.635,and the variation ranged from 0.168 to 0.870,indicating that the local cultivars of Shanxi sorghum have rich polymorphism.The analysis of genetic diversity of 11 different geographical populations showed that the genetic differentiation level was different,and there were different levels of gene flow among populations.Regardless of the number of alleles,the number of effective alleles or the Shannon’s information index,it can be shown that the genetic diversity of the Xinzhou population is the highest,while that of the Yangquan population is the lowest.According to the analysis of genetic distance among populations,the genetic distance between Xinzhou population and Jinzhong population is the smallest,revealing the genetic relationship between the two groups is relatively close,with more frequent gene flow.The genetic distance between Yangquan population and all other populations is relatively large,indicating that the genetic barrier between Yangquan and others.Both the model-based genetic structure analysis and the genetic distance-based N-J clustering analysis divided germplasm materials into two clusters.The genetic relationship between sorghum resources in different regions was not significantly correlated with their geographic distribution.The purpose of this study is to provide theoretical basis for the collection,identification and innovation of sorghum germplasm resources.
作者
李萌
秦慧彬
王宇楠
闫建俊
穆志新
Li Meng;Qin Huibin;Wang Yunan;Yan Jianjun;Mu Zhixin(Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan,030031;Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan,030031)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第18期6068-6076,共9页
Molecular Plant Breeding
基金
山西省基础条件平台项目(201705D121019-5)
山西省农业科学院特色农业技术攻关项目(YGG17061)共同资助。
关键词
SSR标记
高粱
遗传多样性
遗传结构
地方品种
SSR marker
Sorghum
Genetic diversity
Genetic structure
Landrace germplasm
