摘要
目的探索一种适用于去除法医物证检验过程中人源性DNA污染的方法。方法选用快速DNA提取试剂盒,通过涂抹制成血液、唾液斑痕检材,分别以伽玛射线和环氧乙烷处理,而后采用两步擦拭法提取样本,进行STR检测。结果环氧乙烷处理组均未检出STR分型;伽玛射线处理组有60%检出完整STR分型,35%检出部分STR分型,5%未检出。结论环氧乙烷对人源性DNA的破坏效果更强,能够有效运用于去除法医物证检验过程中人源性DNA的污染。
Objective To explore a method for removing human-sourced DNA contamination during forensic evidence analysis.Methods The samples of human blood and oral stains were made from same person through one kit of rapid DNA extraction,respectively treated with gamma ray radiation and ethylene oxide saturation.Afterwards,the samples were respectively wiped onto both a dry swab and wet one to collect the cells,from which the yielded DNA was to have its STRloci profiling carried out.Results No STR genotype was detected from the ethylene oxide treatment group,while complete STR genotype was exposed from 60%of the samples treated with gamma ray radiation,with the rest 35%showing partial STR genotype and the remained 5%none.Conclusions Ethylene oxide is more effective in destroying human DNA,capable of removing human-sourced DNA contamination during forensic evidence analysis.
作者
钟巧梅
黄利维
罗冰科
ZHONG Qiaomei;HUANG Liwei;LUO Bingke(Judicial Firm of Forensic Identifi cation,Wuhan Ruiboxiang Biotechnology Co.,Ltd.,Wuhan 430000,China)
出处
《刑事技术》
2020年第5期507-509,I0008,I0009,共5页
Forensic Science and Technology
