摘要
研究不同提取方法对山楂叶多糖提取率和抗氧化活性的影响,为进一步开发利用山楂叶多糖提供技术依据.分别采用热水浸提法、超声波辅助水提法、微波辅助水提法提取山楂叶多糖.试验结果表明:热水浸提法提取山楂叶多糖的最适条件为料液比1∶30(m∶V)、提取温度90℃,多糖提取率为5.45%;超声波辅助水提法提取山楂叶多糖最适条件为料液比1∶30(m∶V)、超声波功率100 W,多糖提取率为6.82%;微波辅助水提法提取山楂叶多糖的最适条件为料液比1∶30(m∶V)、微波功率300 W,多糖提取率为7.68%.微波辅助水提法提取山楂叶多糖的效果最好,明显优于超声波辅助水提法和热水浸提法.同时以DPPH自由基清除能力,·OH自由基清除能力,Fe^3+还原力为评价指标,研究不同提取方法对山楂叶多糖体外抗氧化活性的影响.结果表明:3种方法提取的粗多糖均具有一定的抗氧化能力,超声波辅助法提取的山楂叶多糖的体外抗氧化活性最高,明显优于微波辅助水提法和热水浸提法.
The effects of different extraction methods on the extraction rate and antioxidant activity of hawthorn leaf are studied,and the technical basis is provided for the further development and utilization of hawthorn leaf polysaccharide.The hot water immersion,ultrasonic-assisted water and microwave-assisted water were used respectively to extract the mountain leaf polysaccharides.The test results show that the most suitable conditions for hot water immersion formulation to extract hawthorn leaf polysaccharides are 1∶30(m∶V),extraction temperature of 90 degrees C,polysaccharide extraction rate of 5.45%,ultrasonic auxiliary water formulation extraction of hawthorn leaf polysaccharide most suitable conditions for the material liquid ratio of 1∶30(m∶V),ultrasonic power 100W,polysaccharide extraction rate of 6.82%,microwave-assisted water formulation extraction of hawthorn leaf polysaccharides the most suitable conditions for the liquid ratio of 1∶30(m∶V),microwave power 300 W,polysaccharide extraction rate of 7.68%.The experimental results show that microwave-assisted water formulation has the best effect of extracting polysaccharides from hawthorn leaves,which is obviously better than ultrasonic-assisted water formulation and hot water immersion.At the same time,the use of hot water immersion,ultrasonic auxiliary water reference,microwave-assisted water reference to the hawthorn leaf coarse polysaccharide extraction,to DPPH free radical removal capacity,·OH free radical removal capacity,Fe^3+reduction force as an evaluation index to study the effect of different extraction methods on the in vitro antioxidant activity of hawthorn leaf polysaccharide.The results showed that the crude polysaccharides extracted by the three methods had certain antioxidant properties,and the in vitro antioxidant activity of the hawthorn leaf polysaccharides extracted by ultrasonic assist method was the highest,which was significantly better than the microwave-assisted water formulation and hot water immersion.
作者
李钊
李宁宁
王宇波
陈松猛
赵圣明
马汉军
LI Zhao;LI Ningning;WANG Yubo;CHEN Songmeng;ZHAO Shengming;MA Hanjun(School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《河南科技学院学报（自然科学版）》
2020年第4期37-44,共8页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
河南省科技攻关项目(202102110060)。
关键词
山楂叶
多糖
提取方式
提取率
抗氧化活性
hawthorn leaves
polysaccharides
extraction
extraction rate
antioxidant activity
