摘要
目的研究青藤碱体外清除氧自由基与抗脂质过氧化的作用。方法超氧阴离子自由基(O2÷)由邻苯三酚自氧化法产生,采用F en ton体系产生羟自由基(.OH),研究青藤碱对O2÷和.OH的清除作用;用硫代巴比妥酸(TBA)法测定VC-F e2+系统诱导大鼠肝匀浆及肝线粒体产生的脂质过氧化物丙二醛(M DA),研究青藤碱抗脂质过氧化的作用。结果青藤碱能清除.OH和O2÷,其EC50分别为1.35和0.136 m g/mL,且呈浓度依赖关系,青藤碱能抑制大鼠肝匀浆自氧化及VC-F e2+系统诱导引起的脂质过氧化。结论青藤碱具有清除氧自由基和抗脂质过氧化的作用,对肝线粒体也有保护作用。
Objective To study the effects of sinomenine on scavenging oxygen free radical and antilipid peroxidation in vitro. Methods Superoxide anion free radical （O2^＋） induced by the auto oxidation of pyrogallol and hydroxyl free radical （·OH） induced by Fenton system were determined by spectrophotometry. The inhibition on homogenate and mitochondrial lipid peroxidation, malondialdehyde （MDA）, of rat liver induced by VC Fe^2＋ system was measured by thiobarabituric acid （TBA） colorimetric method. Results Sinomenine could significantly scavenge O2^＋ and · OH, it＇s EC50 were 1.35 and 0.136 mg/mL respectively. Sinomenine could also remarkably inhibit the lipid peroxidation of rat liver homogenate and mitochondria induced by auto-oxidation and VC-Fe^2＋ system. The scavenging and inhibitory effect exhibit ed in a concentration-dependent manner. Conclusion The results indicate that sinomenine possesses the effect of scavenging oxygen free radicals and antiqipid peroxidation, which shows the protection of liver mitochondria.
出处
《中草药》
CAS
CSCD
北大核心
2006年第1期84-87,共4页
Chinese Traditional and Herbal Drugs
基金
湖北省自然科学基金资助课题(2001ABB156)
关键词
青藤碱
氧自由基
脂质过氧化
sinomenine
oxygen free radical
lipid peroxidation
