摘要
【目的】为了探明提高农林植物耐盐性途径和方法。【方法】对400 mM NaCl处理下的冰叶日中花幼苗,施用不同浓度硝普钠(SNP,外源NO供体,50、100、300、500、800μM),而后检测叶片H2O2、丙二醛(malondialdehyde,MDA)、可溶性糖和脯氨酸含量,以及过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和抗坏血酸过氧化物酶(ascorbate peroxidase,APX)活性。【结果】结果表明,外源SNP提高了盐胁迫下抗氧化酶活性,促进了可溶性糖和脯氨酸的积累,降低了H2O2和丙二醛含量,显著提高了冰叶日中花的耐盐性。【结论】由此可见,NO在冰叶日中花对盐胁迫的生理响应中可能起着重要的信号调控作用。
【Objective】 This study aims to explore the pathways and methods to improve salt tolerance of agricultural and forestry plants.【Methods】 In this study, under 400 mM NaCl the Mesembryanthemum crystallinum seedlings were treated with different levels of SNP(exogenous NO donor, 50 μM, 100 μM, 300 μM, 500 μM, and 800 μM), then the contents of H2O2, malondialdehyde(MDA), proline, and soluble sugar were measured, as well as the activities of peroxidase(POD), superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX). 【Results】 The results showed that exogenous SNP increased the activities of antioxidant enzymes, promoted the accumulation of proline and soluble sugar, and reduced the contents of MDA and H2O2 in the leaves of M. crystallinum under salt stress. 【Conclusion】 The results suggested that NO played an important role in physiological response of M. crystallinum to NaCl stress.
作者
王文果
陈志新
石俊华
胡增辉
WANG Wenguo;CHEN Zhixin;SHI Junhua;HU Zenghui(College of Landscape,Beijing University of Agriculture,Beijing 102206,China;Forestry Survey and Design Institute of National Forestry and Grassland Administration,Beijing 102206,China)
出处
《北京农学院学报》
2020年第4期102-107,共6页
Journal of Beijing University of Agriculture
基金
北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20180509)
国家自然科学基金项目(31201645)。
关键词
冰叶日中花
生理响应
一氧化氮
NACL胁迫
Mesembryanthemum crystallinum
physiologcial response
nitric oxide
NaCl stress
