摘要
简单重复序列(SSR)标记以其高效、可重复和近缘物种高效利用率的优点成为遗传多样性及分子育种研究利用的主要技术手段。苦荞(Fagopyrum tataricum)作为重要的杂粮作物,其分子标记研究也受到了关注。本试验以苦荞‘黑丰1号’籽粒为材料,利用三代测序技术进行转录组测序,基于Perl程序批处理方法筛选SSR位点并开发SSR标记,结果表明:转录组测序共获得15 592条高质量去冗余序列,共鉴定出1 107个SSR位点;按其在染色体中均匀分布原则设计132对genic-SSR引物,其中11对引物具有多态性,多态信息含量(PIC)在0.14~0.60之间;群体结构和聚类分析表明, 123份苦荞种质被划分为2个亚群。上述结果拟为有效利用转录组数据、充分挖掘有效SSR标记,以及苦荞分子标记辅助育种提供理论和实践参考。
Simple sequence repeat(SSR) markers have become one of the main technical methods for the analysis of genetic diversity and molecular breeding due to their high efficiency, repeatability and efficient applications in related species. Tartary buckwheat(Fagopyrum tataricum) is an important minor crop with high level of nutrients and attention has been paid to the development of its molecular markers. In this study, the developing grains of tartary buckwheat variety ‘Heifeng No. 1’ were used as materials for transcriptome sequencing by the third-generation sequencing technique, from which SSR loci were screened based on Perl and SSR markers were developed. The results show that a total of 15 592 high quality sequences were obtained and 1 107 SSRs were identified. A total of 132 pairs of genic-SSR primers were designed. Eleven out of 132 pairs of primers showed polymorphic bands, and polymorphism information content(PIC) was ranged from 0.14 to 0.60. Population structure and cluster analysis showed that 123 tartary buckwheat germplasm were divided into two clusters. The above experiments are intended to provide theoretical and practical references for the effective use of transcriptome data, the exploring of effective SSR markers, and the subsequent marker-assisted breeding in tartary buckwheat.
作者
杜伟
王东航
侯思宇
韩渊怀
李红英
刘龙龙
马名川
王俊珍
孙朝霞
DU Wei;WANG Donghang;HOU Siyu;HAN Yuanhuai;LI Hongying;LIU Longlong;MA Mingchuan;WANG Junzhen;SUN Zhaoxia(Institute of Agricultural Bioengineering,College of Agriculture,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;Institute of Germplasm Resources,Shanxi Academy of Agricultural Science,Taiyuan 030031,China;Xichang Agricultural Institute of Liangshan,Xichang,Sichuan 615000,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2020年第7期1432-1444,共13页
Plant Physiology Journal
基金
国家重点研发计划中欧政府间合作项目(2017YFE-0117600)
山西省农业科学院应用基础研究计划项目(YGC2019FZ2)
国家燕荞麦产业体系项目(CARS-07-A-2)
山西省应用基础研究项目(201801D221296)
山西省回国留学人员科研资助项目(2017-069)。
关键词
三代测序
SSR标记
群体结构
遗传多样性
the third-generation sequencing
SSR marker
population structure
genetic diversity
