摘要
pH稳态对于维持活细胞细胞器的正常功能具有重要作用.细胞器内pH稳态被打破会导致细胞器功能的紊乱,进而引发癌症、神经退行性疾病等相关疾病.因此,在活细胞水平上定量测定pH并对其波动进行实时监测对于理解相关疾病的发生机制非常重要.基于非侵入、高时空分辨率成像的优势,荧光探针非常适合用于活细胞内pH的检测.本综述总结了近些年利用不同种类荧光纳米探针对不同细胞器进行pH成像的研究工作,并对荧光纳米探针应用面临的机遇与挑战进行了展望.
pH homeostasis plays a significant role in maintaining the normal functions of different organelles in living cells.The disruption of pH homeostasis will result in the disorder of organelle function and further lead to the relevant diseases,such as cancer,neurodegenerative disease,etc.Therefore,it is important to quantitively detect pH and monitor the fluctuation of organelle pH in living cells to explore the pathology of relevant diseases.Fluorescent probes are suitable for pH detection in living cell based on the advantages of noninvasive and high spatiotemporal resolution imaging.In this review,we summarized the recently developed fluorescent nanoprobes for the pH detection of different organelles.The chances and challenges of the practical application of fluorescent nanoprobes were also discussed.
作者
公少华
张霞
李娜
唐波
GONG Shaohua;ZHANG Xia;LI Na;TANG Bo(College of Chemistry,Chemical Engineering and Materials Science,Key Laboratory of Molecular and Nano Probes,Ministry of Education,Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong,Institute of Molecular and Nano Science,Shandong Normal University,Jinan 250014,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2020年第9期1933-1944,共12页
Chemical Journal of Chinese Universities
基金
国家重点研发计划项目(批准号:2019YFA0210100)
国家自然科学基金(批准号:91753111,21927881,21874086,21775094)
山东省重点研发计划项目(批准号:2018YFJH0502)
山东省高等学校青年创新科技项目(批准号:2019KJC022)资助.
关键词
荧光纳米探针
细胞器
PH
检测
成像
Fluorescent nanoprobe
Organelles
pH
Detection
Imaging
