摘要
目的研究苋科(Amarathaceae)植物牛膝(怀牛膝,Achyranthes bidentata Bl.)地上部位所含化学成分。方法植物材料用70%乙醇加热回流提取;提取物用Diaion HP-20、MCI Gel CHP-20P、Toyopearl HW-40、Sephadex LH-20、ODS、硅胶等柱色谱结合半制备高效液相色谱进行分离;分离得到的化合物结构根据MS、1 H NMR及13C NMR数据进行鉴定。结果从怀牛膝地上部分分离鉴定了21个化合物:haploperoside A(1)、sweroside(2)、1′-O-苄基-α-L-吡喃鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷(3)、2-乙基-3-甲基-马来酰亚胺-N-β-D-葡萄糖苷(4)、3-ethylidene-4-methyl-2,5-pyrrolidinedione(5)、5-羟基吡啶-2-甲酸甲酯(6)、pericampylinone A(7)、丁香脂素(8)、表丁香脂素(9)、原儿茶酸甲酯(10)、4-羟基苯丙酮(11)、3,4-二羟基苯乙酸甲酯(12)、4-羟基苯乙醇(13)、香草醇(14)、二氢芥子醇(15)、4-羟基苯甲酸(16)、咖啡酸(17)、3-(4-hydroxy-3,5-dimethoxyphenyl)propane-1,2-diol(18)、methyl(E)-3-(4-hydroxyphenyl)acrylate(19)、反式阿魏酸(20)和香草酸(21)。结论化合物1~7、9~19为首次从怀牛膝中分离得到。
Objective To investigate the chemical constituents in the the above-ground part of the plant Achyranthes bidentata Bl.in Amarathaceae.Methods The raw materials were extracted by heating with 70%ethanol,and the extract was prepared by column chromatography on Diaion HP-20,MCI Gel CHP-20P,Toyopearl HW-40,Sephadex LH-20,ODS and silica gel,coupled with the semi-preparative HPLC,to isolate the chemical constituents.The isolated compounds were identified according to their MS,1H NMR and 13C NMR data.Results Twenty one compounds were isolated and identified:haploperoside A(1),sweroside(2),1′-O-benzyl-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(3),2-ethyl-3-methyl-maleimide-N-β-D-glucopyranoside(4),3-ethylidene-4-methyl-2,5-pyrrolidinedione(5),methyl-5-hydroxy-2-pyridinecarboxylate(6),pericampylinone A(7),syringaresinol(8),episyringaresinol(9),methyl 3,4-dihydroxybenzoate(10),4-hydroxyphenylacetone(11),3,4-dihydroxyphenylacetic acid methyl ester(12),2-(4-hydroxyphenyl)-ethanol(13),homovanillyl alcohol(14),dihydrosinapyl alcohol(15),4-hydroxybenzoic acid(16),caffeic acid(17),3-(4-hydroxy-3,5-dimethoxyphenyl)propane-1,2-diol(18),methyl(E)-3-(4-hydroxyphenyl)acrylate(19),trans-ferulic acid(20)and vanillic acid(21).Conclusion Compounds 1-7 and 9-19 were isolated from the title plant for the first time.
作者
徐志贫
王彦志
郭燕
宋志敏
李建鹏
冯卫生
XU Zhi-pin;WANG Yan-zhi;GUO Yan;SONG Zhi-min;LI Jian-peng;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Re-spiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)
出处
《国际药学研究杂志》
CAS
北大核心
2020年第6期450-455,共6页
Journal of International Pharmaceutical Research
基金
河南省重大科技专项资助项目(14104473-2018)
河南中医药大学科技创新团队、科技创新人才计划资助项目(2015XCXTD02)
河南中医学院省属高校基本科研业务专项支持计划(2014KYYWF-ZZCXZ-01)。
关键词
牛膝
化学成分
分离鉴定
Achyranthes bidentata
chemical constituents
isolation and identification
