摘要
目的探讨T细胞免疫球蛋白粘蛋白-3(T cell immunoglobulin mucin-3,Tim-3)和半乳糖凝集素-9(Galectin-9)在宫颈癌临床病理特征中的表达水平及其相关性。方法收集2015年6月-2019年2月新疆医科大学第一附属医院妇科病房收治的确诊为宫颈癌患者63例(宫颈癌组),子宫颈鳞状上皮内病变患者47例(SIL组),慢性宫颈炎患者35例(慢性宫颈炎组),用流式细胞术检测各组外周血CD4^+T细胞表面上的Tim-3、单核细胞上Galectin-9表达情况并对比各组外周血中Tim-3、Galectin-9表达变化,分析宫颈癌患者各临床病理特征中Tim-3、Galectin-9表达情况。结果与慢性宫颈炎组、SIL组相比,宫颈癌组Tim-3^+CD4^+T、Galectin-9^+M表达水平显著增加(P<0.05),宫颈癌III-IV期患者外周血Tim-3^+CD4^+T、Galectin-9^+M的表达水平明显高于I~Ⅱ期的表达水平,病理分级为低分化的宫颈癌患者中Tim-3^+CD4^+T、Galectin-9^+M表达水平明显高于高分化、中分化的表达水平,差异有统计学意义(P<0.05)。手术治疗的宫颈癌患者术前、术后外周血中Tim-3^+CD4^+T、Galectin-9^+M的表达水平的差异有统计学意义(P<0.05)。结论宫颈癌患者外周血中的Tim-3、Galectin-9表达水平明显增高,Tim-3、Galectin-9的表达水平与宫颈癌的临床分期、病理分级特征相关,可能成为临床中评估宫颈癌患者预后的重要指标之一。
Objective To investigate the expression levels of T cell immunoglobulin mucin-3(Tim-3)and Galectin-9 in the clinicopathological characteristics of cervical cancer,and to analyze their correlation.MethodsFrom June 2015 to February 2019,63 patients with cervical cancer(cervical cancer group),47 with cervical squamous intraepithelial lesions(SIL group)and 35 with chronic cervicitis admitted to the Gynecological Ward of the First Affiliated Hospital of Xinjiang Medical University were collected.Flow cytometry was used to detect the expression of Tim-3 on the surface of CD4^+T cells in peripheral blood,and Galectin-9 on monocytes,and to compare the changes of Tim-3,Galectin-9 expression in the blood of each group,and to analysis Tim-3,Galectin-9 expression in various clinicopathological features of patients with cervical cancer.ResultsCompared with the chronic cervicitis group and SIL group,the expression levels of Tim-3^+in CD4^+T and Galectin-9^+M in the cervical cancer group were significantly increased(P<0.05).The expression level of peripheral blood Tim-3^+in CD4^+T and Galectin-9^+M in patients with cervical cancer in stage III-IV was significantly higher than those in stage I-II,and the expression level of Tim-3^+in CD4^+T and Galectin-9^+M in patients with pathological grade of low differentiation was significantly higher than those of high differentiation and middle differentiation(P<0.05).The levels of Tim-3^+CD4^+T and Galectin-9^+M in peripheral blood of patients with cervical cancer before and after surgery were statistically significant(P<0.05).ConclusionThe expression levels of Tim-3 and Galectin-9 in peripheral blood of patients with cervical cancer are significantly increased,and the expression levels of Tim-3 and Galectin-9 are related to the clinical stage and pathological grading characteristics of cervical cancer,which may become one of the important indicators to evaluate the prognosis of cervical cancer patients in clinic.
作者
杨艳
董頔
薛茜文
张旭娟
赵雅文
陈志芳
YANG Yan;DONG Di;XUE Qianwen;ZHANG Xujuan;ZHAO Yawen;CHEN Zhifang(Department of Gynecology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China)
出处
《新疆医科大学学报》
CAS
2020年第8期1004-1008,共5页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81660475)
省部共建中亚高发病成因与防治国家重点实验室立项项目(SKL-HIDCA-2019-8)。
