摘要
目的:研究在复合裂解方式下裂解温度和裂解时间对MCF-7细胞电化学行为的影响.方法:采用电化学法对细胞裂解液进行单线扫描,对比分析裂解温度和裂解时间对细胞裂解液电化学信号的影响,分析裂解温度和裂解时间对电化学法检测结果的影响.结果:随着裂解温度的升高,裂解时间的增长,细胞内的两个电化学信号都是先增加后减小.这两个电化学信号可以分别归属为黄嘌呤和鸟嘌呤、腺嘌呤和次黄嘌呤的混合氧化峰,综合考虑两个混合氧化峰的变化趋势,可以发现实验裂解温度为50℃、裂解时间为30min时,电化学信号最强.结论:不同的裂解条件对检测到的电化学信号有较大影响,裂解温度为50℃、裂解时间为30min时可以作为细胞进行电化学检测的最佳裂解条件.
Objective:To study the effects of pyrolysis temperature and pyrolysis time on the electrochemical behavior of McF-7 cells under the compound pyrolysis method.Methods:The cell lysates were scanned by electrochemical method,and the influence of lysis temperature and lysis time on the electrochemical signal of cell lysates and the detection result by electrochemical method was analyzed.Results:With the increase of lysis temperature and the increase of lysis time,the two electrochemical signals in the cell increased first and then decreased.These two electrochemical signals can be classified as the mixed oxidation peaks of xanthine and guanine,adenine and hypoxanthine respectively.Considering the change trend of these two mixed oxidation peaks,it can be found that the electrochemical signal is the strongest when the experimental pyrolysis temperature is 50℃and the pyrolysis time is 30 min.Conclusion:Different cleavage conditions have great influence on the detected electrochemical signal.The cleavage temperature of 50℃and the cleavage time of 30 min can be used as the optimal cleavage conditions for electrochemical detection of cells.
作者
乔勇昌
崔雨薇
郭爽
王可欣
熊飞
崔继文
王书红
QIAO Yong-chang;CUI Yu-wei;GUO Shuang;WANG Ke-xin;XIONG Fei;CUI Ji-wen;WANG Shu-hong(School of Pharmacy,Jiamusi University,Jiamusi 154007,China;School of Foreign Languages,Jiamusi University,Jiamusi 154007,China)
出处
《黑龙江医药科学》
2020年第4期5-7,共3页
Heilongjiang Medicine and Pharmacy
基金
国家级大学生创新训练项目课题,编号:201810222028
佳木斯大学校管课题,编号:L2014-004
黑龙江省自然科学基金,编号:LH2019H058和QC2017119。
