摘要
为了评价壬基酚(NP)的水环境生态风险,研究了不同浓度壬基酚对小球藻(Chlorella vulgaris)生理及光合特性的影响。设置了0. 635 mmol/L、1. 905 mmol/L、3. 175 mmol/L和4. 445 mmol/L 4个NP浓度梯度和1个对照组,测定了藻液细胞密度、叶绿素a含量、叶绿素荧光参数(Fv/Fm、φPSⅡ、Ik、α和ETRmax)、可溶性蛋白含量、超氧化物歧化酶活性、过氧化氢酶活性及丙二醛含量,探讨了NP影响小球藻生长的机制。结果表明:低浓度NP(0~1. 905 mmol/L)对小球藻的生长及光系统Ⅱ具有促进作用;NP浓度超过1. 905 mmol/L时,随NP浓度升高,小球藻生长和光合活性受到明显的抑制,藻细胞叶绿素a、可溶性蛋白质含量逐渐下降,而丙二醛含量和超氧化物歧化酶活性持续增高。研究表明,不同浓度的NP对小球藻的生理和光合活性产生了一定程度的毒性效应,进一步证实了NP对小球藻生长的影响呈现"Hormesis效应"。
The present paper intends to make an evaluation of the water environment ecological risk of nonylphenol(NP) by way of exposing chlorella vulgaris to the different NP concentration rates of 0.635 mmol/L,1.905 mmol/L,3.175 mmol/L and 4.445 mmol/L.And,then,6 days inoculation later,observation and investigation can be done to trace the changes that have taken place with the contents of chlorophyll a,the malondialdehyde and the soluble protein of C.vulgaris.Furthermore,we have also made efforts to identify and examine the photosynthetic efficiency(the Fv/Fm,φPS Ⅱ,Ik,α and ETRmax),along with the activities of the superoxide dismutase(SOD) and catalase and their corresponding effects on the growth of C.vulgaris.The results of our investigation and examination indicate that it would be possible for the low NP concentration rate(0-1.905 mmol/L) to promote the growth rate and change the function of C.vulgaris of photosynthetic system Ⅱ.However,the growth of C.vulgaris can be obviously inhibited when the NP concentration rate turns to be higher than 1.905 mmol/L.And,then,in case the Fv/Fm of C.vulgaris increases to the highest rate under the stress of 0.635 mmol/L NP,which tends to go up by 15.1% higher than that of the control group,the Fv/Fm may tend to decrease with the increase of NP concentration rate.However,NP had little effect on φPS Ⅱ of C.vulgaris,which is just 0.28 in the 1.905 mmol/L exposure group,merely slightly higher than 0.25 in the control group,with its lowest rate being 0.23 in the 4.445 mmol/L NP exposure group.On the other hand,the effect of NP on the Ik and α indicate that the low concentration rates of NP(0.635 mmol/L and 1.905 mmol/L) can improve the high light intensity tolerance and utilization of C.vulgaris.And,therefore,the above said results can demonstrate that the inhibitory effects of NP on C.vulgaris can be enhanced with the increase of NP concentration rate.For example,the malondialdehyde content and the activity of the superoxide dismutase in C.vulgaris can be increased to 4.15 times and 10.73 times,respectively,in comparison with the control group in case the NP remains at the level of 4.445 mmol/L.And,at the same time,the content rate of chlorophyll a and that of the soluble proteins tend to decrease with the increase of NP concentration rate from 1.905 mmol/L to 4.445 mmol/L.Thus,the results of our experiment prove that the toxic effects shown by NP on the physiological and photosynthetic characteristics of C.vulgaris in a concentration rate should be in a dependent manner when the concentration rate of NP becomes over 1.905 mmol/L.Therefore,the experimental results shown above have further proven that the effect of NP on the growth of C.vulgaris is consistent with that of "Hormesis".
作者
吴义诚
吴文惠
陈国元
傅海燕
王泽杰
WU Yi-cheng;WU Wen-hui;CHEN Guo-yuan;FU Hai-yan;WANG Ze-jie(School of Environment Science and Engineering,Xiamen University of Technology,Xiamen 361024,Fujian,China;College of Environmental Science and Engineering,Qilu University of Technology(Shandong Academy of Science),Jinan 250353,China)
出处
《安全与环境学报》
CAS
CSCD
北大核心
2020年第3期1185-1190,共6页
Journal of Safety and Environment
基金
福建省自然科学基金项目(2019J01848)
厦门市科技计划指导性项目(3502Z20179029)
厦门理工学院科学攀登计划项目(XPDKQ18032)。
关键词
环境学
壬基酚
小球藻
生长抑制
抗氧化系统
光合特性
environmentalology
nonylphenol
Chlorella vulgaris
growth inhibition
antioxidant system
photosynthetic characteristics
