摘要
【目的】研究水稻剑叶叶绿素和光合特性及相关基因对低氮胁迫的响应,为提高水稻对氮肥的吸收和利用效率奠定分子基础。【方法】利用Agilent 4×44K芯片全基因组研究低氮胁迫处理下,2个不同叶绿素含量水稻齐穗期剑叶的光合相关基因表达的变化。【结果】SN19-6和丰锦剑叶的叶绿素含量和净光合速率在低氮胁迫下均有所下降,但SN19-6 2个指标下降的幅度明显较小。低氮胁迫处理与对照处理相比,超绿水稻SN19-6剑叶共有41个光合相关基因表达发生变化(14个在转录水平上下调表达,27个在转录水平上上调表达)。丰锦剑叶有29个光合相关基因表达发生变化(15个在转录水平上下调表达,14个在转录水平上上调表达)。低氮胁迫响应光合相关基因表现出品种特异性,超绿水稻SN19-6有29个为特异响应的,丰锦有17个特异响应的。2水稻品种(系)低氮胁迫响应的光合相关基因有12个重叠的,其中,5个在转录水平上上调表达,7个下调表达。【结论】在低氮胁迫下,水稻剑叶光合相关基因的表达发生变化,不同叶绿素含量水稻品种(系)中的表达既表现特异性,也存在部分重叠。
[ Objective ] The response of photosynthesis-related genes to low nitrogen stress were researched, thus providing a molecular basis for improving the absorption efficiency and utilization efficiency of nitrogen fertilizer in rice. [Method] The Agilent rice genome arrays were used to study the expression change of photosynthesis-related genes in two rice cultivars with different chlorophyll contents under low nitrogen fertilizer stress. [Result] The results showed that chlorophyll content and photosynthetic rate of the flag leaf were decreased under low nitrogen stress in SN19-6 and Fengjin, but the degree of decrease was significantly different between SN19-6 and Fengjin. A total of 41 photosynthesis-related genes （14 photosynthesis-related genes were down-regulated and 27 photosynthesis-related were up-regulated at the transcription level） in flag leaf of supper-green rice SN19-6 and 29 photosynthesis-related genes （15 photosynthesis-related genes were down-regulated and 14 photosynthesis-related genes were up-regulated at the transcription level） in flag leaves of Fengjin were affected by low nitrogen stress. The photosynthesis-related genes for expressed cultivar special-response to low nitrogen stress, with 29 photosynthesis-related genes for SN19-6 and 17 for Fengjin. There was an overlap of photosynthesis-related genes in response to low nitrogen stress between SN19-6 and Fengjin, with 5 up-regulated and 7 down-regulated at the transcription level, respectively. [ Conclusion] Expression of photosynthesis-related genes were affected by low nitrogen stress in flag leaf, and there are genes of cultivar special-response as well as overlap between SN19-6 and Fengjin,
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第1期1-8,共8页
Scientia Agricultura Sinica
基金
公益性行业(农业)科研专项(nyhyzx07-001)
国家"十一五"科技支撑计划(2006BAD01A01-6)
辽宁省科学技术计划重大项目(2008201002)
关键词
水稻
剑叶
基因芯片
光合相关基因
叶绿素含量
光合速率
rice （Oryza sativa L.）
flag leaf
microarray
photosynthesis-related genes
chlorophyll content
photosynthetic rate
