摘要
利用As(Ⅲ)与核酸适配体发生特异性结合使银纳米粒子(AgNPs)在盐溶液中发生不同程度的聚集现象,溶液颜色和光学性质发生改变,据此建立一种新型生物传感器检测重金属As(Ⅲ)。研究表明,在最佳优化条件下,该检测体系在5~100 ng/mL范围内A 650/A 420与As(Ⅲ)浓度呈良好的线性关系(R2=0.9937),检出限为4 ng/mL。方法对As(Ⅲ)具有良好的选择性和灵敏度,将其应用于水样品中As(Ⅲ)的检测,回收率范围为83.31%~96.47%。
In this work,we prepared silver nanoparticles(AgNPs)coated by nucleic acid aptamer.The specific interaction between aptamer and arsenic(Ⅲ)leaded to AgNPs aggregation,which changed the absorption of AgNPs.On this basis,we developed a method to detect arsenic.Under the optimal conditions,the detection system showed a good linear relationship between A 650/A 420 and As(Ⅲ)concentration in the range of 5-100 ng/mL(R2=0.9937),and the detection limit was 4 ng/mL.This method was applied to the detection of arsenic in tap water samples,and the recoveries were in the range of 83.31%-96.47%.
作者
陈倩倩
顾翔源
杨泽怡
张瑞
陆姗姗
朱颖越
CHEN Qianqian;GU Xiangyuan;YANG Zeyi;ZHANG Rui;LU Shanshan;ZHU Yingyue(School of Biotechnology and Food Engineering,Changshu Institute of Technology,Changshu 215500)
出处
《分析科学学报》
CAS
CSCD
北大核心
2020年第3期435-438,共4页
Journal of Analytical Science
基金
国家自然科学基金(No.51602029)
苏州市科技计划(No.SNG201617)
江苏省“六大人才高峰”第十二批高层次人才选拔培养项目(NY-021)
2018年青蓝工程项目。
