摘要
目的:确定瘦素对雄激素性脱发小鼠毛囊的影响。方法:40只雄性C57小鼠随机分组,空白组10只不予处理,余30只背部皮下注射丙酸睾酮5 mg/kg·d进行造模。造模成功后分为3组,继续给药4周:模型组(皮下注射丙酸睾酮)、米诺地尔组(注射丙酸睾酮+外用5%米诺地尔酊)、瘦素组(注射丙酸睾酮+重组小鼠瘦素),比较生长期与休止期毛囊数量比值(A/T),毛囊细胞增殖与凋亡情况及雄激素受体(AR)蛋白含量。结果:(1)米诺地尔组及瘦素组较模型组脱发面积缩小;(2)米诺地尔组与瘦素组较模型组的生长期/休止期毛囊数比值、Ki67表达均明显增加(均P<0.05),两者在米诺地尔组与瘦素组之间比较均无明显统计学差异(均P>0.05);(3)毛囊凋亡数量及AR水平在米诺地尔组与瘦素组间比较均无明显统计学差异(均P>0.05)。结论:皮下注射瘦素对雄激素性脱发模型小鼠有明确治疗作用,且效果与外用5%米诺地尔溶液作用相当。
Objective:To determine the effect of leptin on androgenetic alopecia.Methods:Forty male C57 mice were randomly assigned.Ten mice were not treated(blank group)and 30 mice were treated with 5 mg/kg·d testosterone propionate injection for 4 weeks to establish the androgenetic alopecia mouse model.Then the mice model were divided into three groups and treated for 4 weeks:model group(testosterone propionate injection),minoxidil group(testosterone propionate injection+topical 5%minoxidil tincture),leptin group(testosterone propionate+restructuring mice leptin).The ratio of the number of hair follicles in the growing period to the number of hair follicles in the resting period(A/T)was assessed.The proliferation and apoptosis and androgen receptor protein content in alopecia was detected.Results:(1)The area of hair loss in the minoxidil group and leptin group was smaller than that in the model group.(2)Compared to the model group,the A/T score and Ki67 level in the minoxidil group and leptin group were significantly higher(P s<0.05),and there was no significant statistical difference between minoxidil group and leptin group(P s>0.05).(3)There were no statistically significant difference in apoptotic expression and AR receptor level between the minoxidil group and leptin group(P s>0.05).Conclusion:Subcutaneous injection of leptin is effective on androgenetic alopecia mice model,and the effect is equivalent to topical 5%minoxidil solution.
作者
温旭红
张高峰
潘伦
刘官智
WEN Xuhong;ZHANG Gaofeng;PAN Lun;LIU Guanzhi(Department of Dermatology,Zigong Fourth People's Hospital,Zigong 643000,China)
出处
《中国麻风皮肤病杂志》
2020年第8期461-466,共6页
China Journal of Leprosy and Skin Diseases
基金
四川省卫生和计划生育委员会科研项目(编号:18PJ419)。
关键词
瘦素
雄激素性脱发
增殖
凋亡
雄激素性受体
leptin
androgenetic alopecia
proliferation
apoptosis
androgen receptors
