摘要
咪唑啉酮类除草剂是一类广谱高效除草剂,其作用靶标是乙酰乳酸合成酶(ALS,acetolactate synthase)。培育抗咪唑啉酮类除草剂水稻品种是防治直播稻田杂草危害的有效途径之一。本研究通过喷施咪唑啉酮类除草剂,从30570份水稻种质资源中,获得1份抗咪唑啉酮类除草剂的水稻新种质,该材料抗性性状稳定、抗性效应明显,序列分析表明其ALS基因编码区第1880位的G/A突变导致第627位氨基酸由丝氨酸改变为天冬酰胺,从而产生抗性。本研究发现的抗除草剂新材料,为选育抗除草剂水稻新品种奠定了种质基础。
Imidazolinones,which target the acetolactate synthase,are deployed as broad spectrum and high-efficiency herbicides in order to control the weeds in agriculture. Breeding for rice varieties with resistant to imidazolinone herbicides is one of the economically effective strategies to control weeds in rice fields. In this study,a new rice germplasm resistant to imidazolinone herbicides was identified from 30570 rice germplasm accessions by spraying imidazolinone herbicides. Testing for resistance in its progeny indicated that this resistance was inheritable. Furthermore,a PCR amplification and sequencing of ALS gene encoding sequence revealed a G/A mutation at position 1880 bp position,which lead to an amino acid alteration from serine(S627)to asparagine(N627)and which might be the causal agent for herbicide resistance. Taken together,this study described an elite germplasm with a practical potential in breeding for new herbicide-resistant rice varieties.
作者
毕俊国
谭金松
刘毅
张安宁
王飞名
刘国兰
余新桥
罗利军
BI Jun-guo;TAN Jin-song;LIU Yi;ZHANG An-ning;WANG Fei-ming;LIU Guo-lan;YU Xin-qiao;LUO Li-jun(Shanghai Agrobiological Gene Center,Shanghai 201106)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第4期804-808,共5页
Journal of Plant Genetic Resources
基金
国家重点研发计划(2017YFD0300103)
上海市水稻产业技术体系(沪农科产字(2019)第3号)
国家青年自然科学基金(31501270)。
关键词
水稻
咪唑啉酮
除草剂
抗性
rice
imidazolinone
herbicide
resistance
