摘要
多年生黑麦草是禾本科冷季型草种,温度胁迫严重影响其分布和产量。转录因子调控基因表达在植物响应逆境胁迫中发挥重要作用。该研究以多年生黑麦草品种雅晴为材料,采用高通量RNA-seq技术,对热(40℃)、冷冻(-10℃)和对照(22℃)处理的样本进行转录因子应答分析,以探索多年生黑麦草转录因子对温度胁迫的响应规律,并筛选抗逆转录因子候选基因。结果显示:(1)共鉴定了694个转录因子unigenes,分属于AP2/ERF、GTF、HSF、MYB、NAC、WRKY、bHLH和bZIP等32个家族。(2)在热和冷冻胁迫下,ERF(AP2/ERF)、MYB、NAC和bZIP家族基因均以上调为主,而WRKY家族则多数下调。HSF、GTF和DREB(AP2/ERF)家族成员大多数受热诱导上调,而多数bHLH家族成员受冷冻胁迫上调。(3)功能富集结果表明,多年生黑麦草差异表达的转录因子基因主要参与植物激素信号转导、昼夜节律和病原体互作等通路。研究表明,多年生黑麦草中与胁迫适应相关的转录因子基因普遍上调表达,而与生长和抗病相关的基因多数下调。该研究筛选到大量抗逆转录因子候选基因,为分子育种提供抗逆基因资源。
Perennial ryegrass(Lolium perenne)is a cold-season gramineous species,and its distribution and yield are severely affected by temperature stress.Transcription factors(TFs)can regulate gene expression,playing important roles in plant acclimation to stress.Here,we used the perennial ryegrass cv Yatsyn to analyze the TF genes and their response patterns under heat(40℃),cold(-10℃),control(22℃)by high-throughput RNA sequencing,and to screen adverse tolerance candidates of TF genes.The results showed(1)694 TFs unigenes were identified,belonging to 32 families such as AP2/ERF,GTF,HSF,MYB,NAC,WRKY,bHLH and bZIP,etc.(2)Upon heat and cold stresses,the gene expressions of most members of the ERF(AP2/ERF),MYB,NAC and bZIP families increased,while the WRKY family decreased.In addition,most members of the HSF,GTF and DREB(AP2/ERF)family genes were up-regulated by heat,while most bHLH family members elevated by cold.(3)The functional enrichment results indicated that temperature-responsive TFs were mainly involved in plant hormone signaling,plant circadian rhythms,and plant-pathogen interaction.In general,the TF genes involved in stress adaptation were usually up-regulated,while most genes involved in growth and disease resistance were down-regulated.This study revealed a corner of the response mechanism of plants to temperature stress at the transcriptional level,and provided substantial candidate tolerance genes for the improvement of perennial ryegrass and other plants.
作者
李鑫雨
何媛
代娅
李彩侠
王艳
马欣荣
LI Xinyu;HE Yuan;DAI Ya;LI Caixia;WANG Yan;MA Xinrong(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;University of Chinese Academy of Sciences, Beijing 100049, China;Innovation Academy for Seed Design, Chinese Academy of Sciences, Chengdu 610041, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第5期773-784,共12页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家科技支撑项目(2014BAC05B04-03)
国家自然科学青年基金(31502003)。
