摘要
目的探讨在中山地区献血者血液筛查中应用核酸检测技术的必要性及效果。方法选用2种酶联免疫吸附试验(ELISA)试剂检测2015年8月至2019年12月中山地区献血者标本,对其中无反应性或单试剂反应性标本进行核酸检测。结果对203836例ELISA双试剂无反应性标本进行核酸检测,共检出核酸反应性标本223例,其中乙型肝炎病毒DNA 220例,丙型肝炎病毒RNA 3例,未检出人类免疫缺陷病毒RNA。对575例ELISA单试剂反应性标本进行核酸检测,共检出18例乙型肝炎病毒DNA反应性标本。ELISA双试剂无反应性标本和单试剂反应性标本的核酸反应率分别为1.09‰和31.30‰,核酸检测Ct均值分别为37.42±18.73和34.36±2.65,差异均有统计学意义(P<0.05)。结论核酸检测技术在献血者血液安全筛查中的应用,可以提高病毒检出率,缩短病毒检测“窗口期”,发现隐匿性病毒感染,降低输血相关病毒感染风险。
Objective To explore the necessity and effect of nucleic acid detection technology in blood screening of blood donors in Zhongshan area.Methods Two kinds of ELISA reagents were used to detect the samples of blood donors in Zhongshan area from August 2015 to December 2019,among which non reactive or single reagent reactive samples were used for nucleic acid detection.Results A total of 223 nucleic acid reactive samples were detected in 203836 ELISA double reagents non reactive samples,including 220 cases of hepatitis B virus DNA,3 cases of hepatitis C virus RNA,and no human immunodeficiency virus RNA was detected.A total of 18 hepatitis B virus DNA reactive samples were detected in 575 ELISA single reagent reactive samples.The reaction rate of nucleic acid was 1.09‰and 31.30‰in the double reagents non reactive samples and the single reagent reactive samples,and the Ct of nucleic acid detection was 37.42±18.73 and 34.36±2.65,respectively,the difference was statistically significant(P<0.05).Conclusion The application of nucleic acid detection technology in blood safety screening of blood donors can improve the detection rate of virus,shorten the"window period"of virus detection,find hidden virus infection,and reduce the risk of blood transfusion related virus infection.
作者
廖艳婷
孙爱农
李乔
LIAO Yanting;SUN Ainong;LI Qiao(Department of Clinical Laboratory,Zhongshan Central Blood Station,Zhongshan,Guangdong 528400,China)
出处
《检验医学与临床》
CAS
2020年第15期2113-2115,2118,共4页
Laboratory Medicine and Clinic
基金
广东省中山市社会公益科技研究项目(2016B1096)。
关键词
献血者
血液筛查
核酸检测
中山地区
blood donors
blood screening
nucleic acid detection
Zhongshan area
