摘要
以响叶杨叶片为外植体进行组培快繁,对外植体消毒、诱导培养、增殖培养、生根培养及炼苗移栽进行了深入研究,结果表明:0.10%升汞溶液最佳消毒时间为5.5 min;最佳诱导培养基为处理1-7(MS+1.00 mg·L-16-BA+0.10 mg·L-1 NAA),诱导率为90.0%;最佳增殖培养基为处理2-8(MS+0.40 mg·L-16-BA+0.15 mg·L-1 NAA),增殖倍数为4.0;适宜生根培养基为1/2 MS+0.10 mg·L-1 NAA,生根率为98.0%;炼苗后移栽选用树皮∶珍珠岩∶黄土=1∶1∶1的基质,成活率达92.0%。研究指出,通过芽苞嫁接、温室培育方法,有利于响叶杨野外采集的外植体脱毒。
Study on tissue culture and rapid propagation of the leaves explants of Populus adenopoda Maxim,the results showed that:the optimum disinfection time of 0.1%mercury solution was 5.5 min;the optimum induction medium was treatment 1-7(MS+1 mg·L-16-BA+0.1mg·L-1 NAA),the induction rate was 90.0%;the optimum multiplication medium was treatment 2-8(MS+0.4 mg L-16-BA+0.15 mg·L-1 NAA),the multiplication rate was 4.0;the optimal root culture medium is 1/2 ms+0.1 mg·L-1 NAA,the rooting rate was 98.0%;after seedling refining,the bark was transplanted∶perlite∶loess soil=1∶1∶1,the survival rate was 92.0%.It was pointed out that the method of bud grafting and greenhouse cultivation was beneficial to the detoxification of explants collected in the field.
作者
梁丽容
付利
张国君
康伟静
宋国宝
肖毅
LIANG Lirong;FU Li;ZHANG Guojun;KANG Weijing;SONG Guobao;XIAO Yi(Tiger Fotest&Paper Group Co.,Ltd.,Yueyang 414002,Hunan,China)
出处
《湖南林业科技》
2020年第3期115-119,123,共6页
Hunan Forestry Science & Technology
关键词
响叶杨
叶片
不定芽
再生植株
Populus adenopoda Maxim
blade
adventitious bud
regenerated plant
