摘要
为优选出大岩桐组织培养中最佳的消毒灭菌方法,以大岩桐幼嫩叶片为试验材料,研究前期75%酒精(10、30、60 s)预处理以及后期0.1%HgCl2、5%NaClO、10%H2O23种不同消毒剂种类分别在(8、10、12 min)处理下对大岩桐生长状况的影响。结果表明,10%H2O2处理下的长菌率和褐化率显著高于0.1%HgCl2和5%NaClO的处理,且长菌和褐化发生时间较早;研究中消毒效果最佳的2个组合为75%酒精处理30 s+0.1%HgCl2处理10 min和75%酒精处理30 s+5%NaClO处理10 min。该研究为与大岩桐类似的其他被子植物的组织培养提供了参考。
In order to optimize the sterilization method in tissue culture of Sinningia speciosa,young leaves of S.speciosa were used as experimental materials.The effects of pretreatment with 75%alcohol(10,30,and 60 s)in the early stage and post-treatment with 0.1%HgCl2,5%NaClO and 10%H2O2(8,10,and 12 min each)on the growth of S.speciosa leaves were studied.The results showed that the pollution rate and browning rate under 10%H2O2 treatment were significantly higher than those of 0.1%HgCl2 and 5%NaClO,and the pollution and browning occurred earlier.The two most effective combinations were 75%alcohol treatment for 30 s+0.1%HgCl2 for 10 min and 75%alcohol treatment for 30 s+5%NaClO for 10 min.This experiment provides a reference for tissue culture of other angiosperms similar to globulus.
作者
杨晨星
李晶
穆立蔷
YANG Chen-xing;LI Jing;MU Li-qiang(Forestry College,Northeast Forestry University,Harbin 150040,Heilongjiang,China;Heilongjiang Academy of Forestry Science,Harbin 150040,Heilongjiang,China)
出处
《西北林学院学报》
CSCD
北大核心
2020年第4期89-94,共6页
Journal of Northwest Forestry University
基金
黑龙江省林业外来物种调查与研究(KJZXSA2019031)。
关键词
大岩桐
叶片
组织培养
消毒时间
消毒剂种类
Sinningia speciose
leaf
tissue culture
sterilization time
disinfectant species
