摘要
目的了解诺如病毒在餐饮服务单位和宾馆住宿场所环境中的分布及厨师隐性感染状况,并对其基因型别进行研究。方法共抽检北京市西城区40家餐饮服务单位和10家宾馆住宿场所,每家单位采集4份环境涂抹样品及2名厨师粪便标本。用实时荧光聚合酶链式反应(PCR)对诺如病毒进行初步鉴定。通过逆转录聚合酶链式反应(RT-PCR)对诺如病毒开放读码框(opening reading frames,ORF)1区序列进行扩增,并对测序结果进行序列和进化分析。结果共采集环境表面涂抹样品200份,厨师粪便标本100份。其中,1份墩布池涂抹样品为诺如病毒阳性,环境样品诺如病毒阳性率为0.5%(1/200),3份厨师粪便中检出诺如病毒,隐性感染率为3.0%(3/100)。3名厨师粪便标本诺如病毒ORF1区序列经RT-PCR扩增,进化树显示,1名厨师为GII.17基因型,2名厨师为GI.3基因型。结论北京市西城区餐饮服务单位及宾馆住宿场所存在诺如病毒传播风险,应强化卫生管理,重视物体表面消毒及厨师健康教育,防止诺如病毒的进一步扩散。
Objective To investigate the prevalence and genotyping of Norovirus in environment and food handlers in catering services and hotels. Methods A total of 40 catering services and 10 hotels were selected as the sampling sites in this study and 4 environment samples and 2 food-handler fecal samples were collected from each site. RNA was extracted and preliminary analyzed for Norovirus by real-time polymerase chain reaction(PCR). Partial opening reading frames 1(ORF1) sequences were amplified by reverse transcription-polymerase chain reaction(RT-PCR), followed by sequence and phylogenetic analysis. Results One mop sink swab out of 200 environment samples(0.5%,1/200) and 3 out of 100 food handlers fecal samples(3.0%,3/100) were positive for Norovirus. The genotyping of Norovirus revealed that one belonged to GII.17 genotype and two belonged to GI.3 genotype. Conclusion The transmission risk of Norovirus in catering services and hotels should be paid more attention to and hygienic management should be strengthened. Health education of food handlers to prevent the transmission of Norovirus should be strengthened.
作者
吉彦莉
王永全
崔海洋
靳博
JI Yanli;WANG Yongquan;CUI Haiyang;JIN Bo(Xicheng Center for Disease Control and Prevention,Beijing 100120,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2020年第3期284-287,共4页
Chinese Journal of Food Hygiene
基金
首都卫生发展科研专项(首发2020-3-7022)。
关键词
诺如病毒
餐饮服务单位
宾馆
厨师
隐性感染
环境表面
基因型
Norovirus
catering service unit
hotel
food handler
asymptomatic infection
environment surfaces
genotype
