摘要
芽孢杆菌是一种重要的微生物资源,被广泛应用于农业生产中。黑龙江八一农垦大学生命科学技术学院微生物研究室分离获得了一株具有产酸能力的短小芽孢杆菌,为明确该菌株最优生长与产酸条件,通过改变碳、氮源及乙醇浓度与培养温度等,利用响应面分析优化菌株生长及产酸发酵条件。结果表明:该菌株最适菌株生长及产酸的碳源和氮源分别为蔗糖和酵母膏,最适乙醇浓度为3%,最适培养温度为30℃。此条件下的OD600为2.190,pH为2.93。
Bacillus is an important microbial resource which is widely used in agricultural production.An acid producing strain of Bacillus pumilus was obtained from the Laboratory of Microbiology,School of Life Science and Technology,Bayi Agricultural University,Heilongjiang Province.In order to determine the optimal growth and acid production conditions of the strain,response surface analysis was used by changing carbon,nitrogen source,ethanol concentration and culture temperature.The results showed that the most suitable carbon and nitrogen sources for the growth and acid production of the strain were sucrose and yeast extract,the most suitable ethanol concentration was 3%,and the most suitable culture temperature was 30℃.Under this condition,OD600 was 2.190 and pH was 2.93.
作者
陈宇峰
严花
李可
孙冬梅
CHEN Yu-feng;YAN Hua;LI Ke;SUN Dong-mei(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Linkou Agricultural Technology Extension Center,Linkou 157600,China)
出处
《黑龙江农业科学》
2020年第5期91-95,共5页
Heilongjiang Agricultural Sciences
基金
黑龙江省农垦总局项目(HKKY190403)
大庆市指导项目(zd-2017-63)。
关键词
短小芽孢杆菌
响应面
生长
产酸
Bacillus pumilus
response surface
growth
acid producing
