摘要
目的:分析胰腺癌小鼠恶病质状态下姬松茸多糖结合硒对代谢的作用,为临床上将姬松茸多糖结合硒应用于改善胰腺癌恶病质状态提供参考。方法:选取40只成功建立小鼠胰腺癌细胞株SW1990皮下移植瘤模型的6~7周龄BALB/c-nu/nu SPF级小鼠,分为A组(模型组)、B组(低剂量组)、C组(中剂量组)、D组(高剂量组),另选取10只未做任何处理的健康小鼠作为E组。各组给药后设置4个观察点,T1(造模后7 d)、T2(造模后14 d)、T3(造模后21 d)、T4(造模后28 d),比较各组小鼠的体重变化、摄食量、饮水量、各血清因子水平、各生化指标水平、肿瘤体积。结果:T1~T2随着肿瘤的增长,各组小鼠的体重逐渐增长,T2结束后次日开始给药,各组小鼠间体重在T3、T4时比较,差异有统计学意义(P<0.05);T2、T3、T4时,A组、B组、C组、D组小鼠的摄食量、饮水量较T1明显降低,且均低于E组;T4时检测各组小鼠的各血清因子水平,A组、B组、C组、D组小鼠的血清IL-1、IL-6、TNF-α、TG、CHOL水平均高于E组,且A组高于B组、C组、D组,并呈现出剂量-反应关系。T4时检测各组小鼠的各生化指标水平,A组、B组、C组、D组小鼠的血清总蛋白、白蛋白、前白蛋白、血红蛋白水平均低于E组,且B组、C组、D组均高于A组,差异均有统计学意义(P<0.05)。T1~T4时,各组小鼠的肿瘤体积均逐渐增大,且各时刻B组、C组、D组均高于A组,(均P<0.05),呈现出剂量-反应关系。结论:姬松茸多糖结合硒可以有效改善胰腺癌小鼠恶病质状态,调节代谢紊乱状态,改善营养状况,提高免疫力。
Objective:To analyze the effect of polysaccharides combined with selenium on metabolism in mice with cachexia of pancreatic cancer,so as to provide reference for clinical application of polysaccharides combined with selenium in improving cachexia of pancreatic cancer.Methods:40 BALB/c-nu/nu SPF mice of 6~7 weeks old with successful establishment of a mouse pancreatic cancer cell line SW1990 subcutaneously transplanted tumor model,which were divided into group A(model group),group B(low dose group),group C(middle dose group)and group D(high dose group),and 10 healthy mice without any treatment were selected as group E.4 observation points were set in each group after administration,T1(7d after modeling),T2(14d after modeling),T3(21d after modeling),T4(28d after modeling),and the body weight,food intake,water intake,serum factor levels,biochemical index levels,and tumor volume of each group of mice was compared.Results:With the growth of tumors in T1~T2,the body weight of mice in each group gradually increased.The administration began the day after the end of T2.The weight of mice in each group was different between T3 and T4(P<0.05).At T2,T3 and T4,the food intake and water consumption of the mice in groups A,B,C,and D were significantly lower than those in T1,and both were lower than those in group E.The levels of serum factors in each group of mice were detected at T4,the serum levels of IL-1,IL-6,TNF-α,TG,and CHOL in group A,group B,group C,and group D were higher than those in group E,and group A was higher than groups B,C,and D,and showed Dose-response relationship.At T4,the levels of various biochemical indicators of the mice in each group were detected.The serum total protein,albumin,prealbumin,and hemoglobin levels of mice in groups A,B,C,and D were lower than those in group E,and group B,Group C and D were higher than group A,and the above differences were statistically significant(P<0.05).From T1 to T4,the tumor volume of mice in each group gradually increased,and at each time,groups B,C,and D were higher than group A,the difference was statistically significant(P<0.05),showing a dose-response relationship.Conclusion:The combination of agaricus matsutake polysaccharide and selenium can effectively improve the cachexia state of pancreatic cancer mice,regulate metabolic disorders,improve nutritional status and improve immunity.
作者
冯义伶
郭仁德
曲雅静
瞿峰
FENG Yi-ling;GUO Ren-de;Qu Ya-jing;QU Feng(Department of Oncology,Characteristic Medical Center of Chinese People s Armed Police Force,Tianjin 300162;Departments of General surgery,Tianjin First Central Hospital,Tianjin 300192;Cancer Institute,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China)
出处
《川北医学院学报》
CAS
2020年第3期357-360,383,共5页
Journal of North Sichuan Medical College
基金
国家自然科学基金(81172103)。
关键词
姬松茸多糖
亚硒酸钠
胰腺癌
恶病质
Agaricus blazei Murrill polysaccharide
Sodium selenite
Pancreatic cancer
Cachexia
