摘要
目的探究DNA结合抑制因子2(ID2)、Rab5基因在子宫内膜异位症(EMS)患者中的表达及其意义。方法以2017年6月至2019年8月本院138例EMS患者为研究组,另120例行输卵管绝育手术患者作为对照组。检测两组子宫内膜组织中Rab5 mRNA、ID2 mRNA表达量,采用受试者工作特征(ROC)曲线分析两者单独及联合的诊断价值。研究组按照r-AFS分期划分,比较不同病情分期患者Rab5 mRNA、ID2 mRNA、临床指标[血清癌胚抗原125(CA125)、子宫内膜抗体(EmAb)]水平,分析Rab5 mRNA、ID2 m RNA与病情分期、血清CA125、EmAb的相关性。结果研究组子宫内膜组织中Rab5 mRNA、ID2 m RNA表达量高于对照组(P<0.05);Rab5 mRNA与ID2 mRNA联合诊断EMS的AUC最大,为0.802,最佳诊断敏感度为78.99%,特异度为69.17%;EMS患者病情分期与Rab5 mRNA、ID2m RNA表达及血清CA125、EmAb水平存在正相关关系(P<0.05);EMS患者Rab5 mRNA、ID2 mRNA表达与血清CA125、EmAb水平存在正相关关系(P<0.05)。结论 EMS患者Rab5 m RNA、ID2 mRNA表达异常增高,与病情分期及血清CA125、EmAb水平关系密切,可辅助临床EMS诊断。
Objective To explore the expression and significance of DNA binding inhibitor 2(ID2)and Rab5 genes in patients with endometriosis(EMS).Methods 138 patients with EMS in our hospi-tal from June 2017 to August 2019 were selected as the study group,another 120 patients who underwent tubal sterilization were selected as the control group during the same period.The expression levels of Rab5 mRNA and ID2 mRNA in the endometrial tissues of the two groups were detected.The receiver operating characteris-tic(ROC)curve was used to analyze the diagnostic value of the two separately and jointly.The research group was divided according to the r-AFS stage.The levels of Rab5 mRNA,ID2 mRNA,and clinical indicators[se-rum carcinoembryonic antigen 125(CA125),endometrial antibody(EmAb)in patients with different disease stages were measured and compared.The correlation between Rab5,ID2 mRNA and disease stage,serum CA125,EmAb was analyzed.Results The expression levels of Rab5 mRNA and ID2 mRNA in endometrial tissue in the study group were higher than those in the control group(P<0.05).Rab5 mRNA and ID2 mRNA combined diagnosis of EMS has the largest AUC of 0.802,the best diagnostic sensitivity is 78.99%,and the specificity is 69.17%There is a positive correlation between the disease stage of EMS patients and the expres-sion of Rab5 mRNA,ID2 mRNA and serum CA125 and EmAb levels(P<0.05).The expression of Rab5 mRNA and ID2 mRNA in EMS patients was positively correlated with serum CA125 and Emab levels(P<0.05).Conclusion The increased Rab5 mRNA and ID2 mRNA expressions in EMS patients are closely relat-ed to the disease stage and the serum levels of CA125 and EmAb which can assist clinical EMS diagnosis.
作者
陈豫中
赖威
张国安
李正英
CHEN Yuzhong;LAI Wei;ZHANG Guoan;LI Zhengying(Department of Obstetrics and Gynecology,951 Hospital of the Chinese peopleundefineds Liberation Army,Korla,Xinjiang,China,841000;The Xinjiang Uygur Autonomous Region people's Hospital,Xinjiang,China,830001)
出处
《分子诊断与治疗杂志》
2020年第6期724-727,732,共5页
Journal of Molecular Diagnostics and Therapy
基金
新疆维吾尔自治区卫生计生适宜技术推广项目(SYTG-201706)。
