摘要
为确保消费者饮茶安全,建立了QuEChERS-GC-MS/MS快速测定金银花和其茶汤中6种有机磷农药(对硫磷、倍硫磷、特丁硫磷、治螟磷、丙溴磷、灭线磷)残留的检测方法。金银花和茶汤分别经乙腈和乙酸乙酯提取、盐析离心、混合净化剂(10 mg MWCNTs、15 mg PSA、150 mg无水硫酸镁)净化、0.22μm滤膜过滤后,供GC-MS/MS检测。结果表明:金银花和茶汤中,有机磷农药在1~1000μg/kg和1~1000μg/L呈现良好的线性关系,R2>0.991;在4个添加水平下,回收率在67.2%~110.3%,相对标准偏差小于19%(n=6);金银花和茶汤中,有机磷农药定量限分别为1μg/kg和1μg/L。在此基础上,深入研究了金银花在冲泡过程中6种有机磷农药的迁移预测模型,表明农药迁移率(TR)与农药的正辛醇-水分配系数(Kow)关联性较大,由此获得预测模型为lg TR=-0.5362 lg Kow+3.9152(lg Kow>3),R2=0.914,拟合度较好;而农药迁移率与水溶解度(Ws)并未表示出关联性。该检测方法和迁移模型的建立将有利于更深层次认知农药化学污染物在金银花浸泡过程中的迁移行为,以期为制定金银花中有机磷农药最大残留限量标准提供科学依据和技术支撑。
In order to ensure the safety of tea drinking for consumers,a rapid method for the determination of six organophosphorus pesticide(parathion,fenthion,terbufos,sulfotep,profenofos and ethoprophos)residues in honeysuckle and tea infusion was established by QuEChERS-GC-MS/MS.Honeysuckle and tea infusion were extracted by acetonitrile and ethyl acetate respectively,centrifugated after salting out,purified by mixed purification agents(10 mg MWCNTs,15 mg PSA and 150 mg MgSO4),filtered through 0.22μm membrane and then detected by GC-MS/MS.The results showed that in honeysuckle and tea infusion,six organophosphorus pesticides showed a good linear relationship in the concentration range of 1-1000μg/kg and 1-1000μg/L,respectively.And the correlation coefficients were all above 0.991.Under four addition levels,the recoveries were between 67.2%and 110.3%,and the RSD was less than 19%(n=6).The limit of quantitation of honeysuckle and tea infusion was 1μg/kg and 1μg/L,respectively.On this basis,the migration model of six organophosphorus pesticides was studied during brewing process of honeysuckle.The results showed that the transfer rate(TR)of organophosphorus pesticides was highly correlated with the octanol-water partition coefficient(Kow).The prediction model was lg TR=-0.5362 lg Kow+3.9152(lg Kow>3),and the correlation coefficient was 0.914,which meant a good fitting degree.However,the correlation between pesticide mobility and water solubility(Ws)was not shown.The establishment of the detection method and migration model will be helpful to further understand the migration behavior of chemical pollutants during brewing process of honeysuckle,and provide scientific basis and technical support for establishing the maximum residue limit of organophosphorus pesticides in honeysuckle.
作者
李建勋
王玉珍
卢嘉
徐迪
单吉浩
范蓓
LI Jianxun;WANG Yuzhen;LU Jia;XU Di;SHAN Jihao;FAN Bei(Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
出处
《食品科学技术学报》
CAS
CSCD
北大核心
2020年第3期119-126,共8页
Journal of Food Science and Technology
基金
农业农村部2019年国家农产品质量安全风险评估项目(GJFP2019015)。
关键词
金银花
茶汤
有机磷农药
检测
迁移模型
honeysuckle
tea infusion
organophosphorus pesticide
detection
migration model
