摘要
翻译起始因子eIF1A是蛋白合成重要的调控因子之一,在植物响应非生物胁迫过程中发挥重要的调控作用。本研究通过克隆获得了1个eIF1A基因MieIF1A-a(GenBank登录号:KP271044),对其进行了信息学分析,并通过表达模式和拟南芥转化来研究其功能,研究结果发现,MieIF1A-a基因的cDNA全长为604 bp,包含1个435 bp开放阅读框(ORF),编码144个氨基酸,分子量为16.45 kD;与葡萄中VveIF1A基因相似性最高;在成熟果实中表达量显著高于其他组织,在盐和干旱胁迫条件下均能诱导该基因在芒果叶片中的上调表达;在盐和干旱胁迫下,与野生型相比,转基因植株的结荚数量多,以及转基因植株生长良好,受影响较小;且在干旱胁迫下,芒果的相对含水量、叶绿素和脯氨酸含量较高,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)酶活性较强,丙二醛(MDA)含量相对较低。综上,本研究初步阐明了MieIF1A-a基因参与芒果果实发育和逆境胁迫调控作用,为后续深入芒果抗逆研究工作奠定了研究基础。
Eukaryotic translation initiation factors 1A(eIF1A)function as important regulatory factors in protein synthesis and play crucial roles in the response to abiotic stresses in plants.In this study,we cloned the translation initiation factor MieIF1A-a(GenBank accession number:KP271044)in mango.Bioinformatic analysis was carried out on the MieIF1A-a,and its function was studied through the expression pattern analysis and Arabidopsis thaliana(L.)Heynh.genetic transformation.Sequences analysis showed that the full length of the MieIF1A-a cDNA was 604 bp with an open reading frame of 435 bp,encoding 144 amino acids,and the molecular weight was 16.45 kD.MieIF1A-a had the highest similarity with VveIF1A of grapevine,had significantly higher expression level in mature fruit than in other tissues.It was up-regulated under salt and drought stresses,causing transgenic plants to produce more pods,to grow better and to be less adversely affected than wild-type plants.Compared with wild-type plants,the relative water content,chlorophyll and proline contents were higher,SOD,POD and CAT activities were stronger,and MDA content was relatively lower in transgenic plants under drought stress.In summary,this study revealed that the MieIF1A-a gene was involved in the regulation of fruit development and responded to stresses,which laid a foundation for further research on stress resistance of mango.
作者
李丽淑
罗聪
安振宇
刘召亮
董龙
何新华
LI Li-shu;LUO Cong;AN Zhen-yu;LIU Zhao-liang;DONG Long;HE Xin-hua(College of Agriculture,Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Nanning 530004;Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007;Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第3期743-752,共10页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31660561)
广西创新驱动发展专项资金资助项目(GXKJ-AA17204097-3,GXKJ-AA17204026-2)
广西重点研发计划项目(GXKJ-AB17292010)
国家现代农业产业技术体系广西芒果创新团队栽培岗位项目(nycytxgxcxtd-06-02)。
