摘要
目的探究长链非编码 RNA MEG (long-chain non-coding RNA-MEG,LNC RNA-MEG) 通过微小 RNA-21-5p (micro RNA-21-5p,miR-21-5p) 调控神经细胞凋亡,进而调控脊髓损伤 (spinal cord injury,SCI) 的作用和机制。方法从 NCBI 数据库中选取大鼠脊髓损伤过程中的基因表达数据和 miRNA 表达数据进行差异表达分析,并预测可能参与脊髓损伤过程的 miRNA。随后,在大鼠脊髓损伤模型上,对生物信息学预测的变化显著的 miRNA 进行 qPCR 验证。使用慢病毒 shRNA-LNC RNA-MEG 处理脊髓损伤大鼠,进一步明确 LNC RNA-MEG 调控脊髓损伤的作用。结果 miR-21-5p 的变化显著。在脊髓损伤大鼠模型中 miR-21-5p 的上游基因 LNC RNA-MEG 的表达量显著上升。慢病毒 shRNA-LNC RNA-MEG 处理后可增加 miR-21-5p 的表达,并减少凋亡相关蛋白的表达,进而抑制神经细胞凋亡。结论 LNC RNA-MEG 能够通过 miR-21-5p 影响大鼠脊髓损伤过程中神经细胞的凋亡作用。
Objective To explore the role and mechanism of long-chain non-coding RNA (LNC RNA) LNC RNA-MEG in spinal cord injury (SCI) by regulating neuronal apoptosis through micro RNA (miRNA) miR-21-5p.Methods Gene expression data and miRNA expression data of SCI in rats were selected from NCBI database for differential expression analysis,and miRNAs that might participate in SCI were predicted.Subsequently,miRNAs with significant changes predicted by bioinformatics were verified by qPCR on a rat SCI model.SCI rats were treated with lentiviral shRNA-LNC RNA-MEG to further clarify the role of LNC RNA-MEG in regulating SCI.Results Changes of miR-21-5p were significant.It was found that the expression of LNC RNA-MEG,an upstream gene of miR-21-5p,increased significantly in SCI rat models.Lentiviral shRNA-LNC RNA-MEG treatment increased the expression of miR-21-5p and reduced the expression of apoptosis-related proteins,thereby inhibiting neuronal apoptosis.Conclusions LNC RNA-MEG regulates neuron apoptosis during SCI in rats through miR-21-5p.
作者
唐一钒
陈竞轩
曹兵
苑博
石维
李凤宁
TANG Yi-fan;CHEN Jing-xuan;CAO Bing;YUAN Bo;SHI Wei;LI Feng-ning(Department of Orthopaedics,Shanghai Changzheng Hospital,Shanghai,200003,China)
出处
《中国骨与关节杂志》
CAS
2020年第5期383-389,共7页
Chinese Journal of Bone and Joint
基金
国家自然科学基金(81702138)。
