摘要
目的通过比较两种不同血管性痴呆(VD)模型大鼠的制作方法,筛选合适的造模方法。方法将32只SD大鼠分为模型Ⅰ组(双侧颈总动脉反复缺血再灌注加左侧永久结扎)、模型Ⅱ组(双侧颈总动脉反复缺血再灌注加双侧永久结扎)、假手术组(只钝性分离颈总动脉,不进行缺血再灌注且不结扎颈总动脉),比较模型Ⅰ组、模型Ⅱ组与假手术组的死亡率以及大鼠学习记忆与空间认知能力。结果造模后48 h和30 d时3组大鼠死亡率比较差异无统计学意义(P>0.05);造模后1周时,两模型组大鼠定位航行试验及空间探索试验结果与假手术组比较差异有统计学意义(P<0.05);造模后4周时,两模型组大鼠定位航行试验结果与假手术组比较差异有统计学意义(P<0.05),模型Ⅰ组与假手术组比较逃避潜伏期差距缩小,模型Ⅱ组与假手术组比较逃避潜伏期差距扩大,且模型Ⅰ组造模4周时逃避潜伏期与造模1周时比较差异无统计学意义(P>0.05);造模后1周时,两模型组大鼠穿越平台次数与假手术组比较差异有统计学意义(P<0.05),模型Ⅰ组与模型Ⅱ组组间比较差异无统计学意义(P>0.05);造模后4周时,模型Ⅱ组大鼠穿越平台次数与假手术组比较差异有统计学意义(P<0.05);造模后1周时,两组模型组大鼠的第一次穿越平台的时间与假手术组比较差异有统计学意义(P<0.05),模型Ⅰ组与模型Ⅱ组组间比较差异有统计学意义(P>0.05);造模后4周时,两模型组大鼠第一次穿越平台的时间与假手术组比较差异有统计学意义(P<0.05),模型Ⅰ组大鼠第一次穿越平台的时间与模型Ⅱ组间比较差异有统计学意义(P<0.05)。造模后4周时模型Ⅱ组与假手术组空间探索实验差距拉大,而模型Ⅰ组与假手术组差距缩小。结论通过反复预实验可以极大地降低大鼠的死亡率,双侧颈总动脉反复缺血再灌注加单侧永久结扎的造模方法更加适合短时间内研究,双侧颈总动脉反复缺血再灌注加双侧永久结扎的造模方法可以运用于血管性痴呆大鼠的长期研究中。
Objective To compare the methods of making two different vascular dementia(VD)mo P<del rats,and to screen suitable modeling methods.Methods Thirty-two SD rats were divided into modelⅠgroup(bilateral common carotid artery repeated ischemia-reperfusion plus left permanent ligation)and modelⅡgroup(bilateral common carotid artery repeated ischemia-reperfusion plus bilateral permanent ligation),Sham operation group(only blunt separation of the common carotid artery,no ischemia-reperfusion and no ligation of the common carotid artery),compare the mortality of modelⅠgroup,modelⅡgroup and sham operation group,and the learning memory and spatial recognition Knowledge ability.Results There was no significant difference in the mortality of the three groups of rats at 48h and 30d after modeling(P>0.05);at 1 week after modeling,the results of the positioning navigation test and space exploration test of the two model groups and the sham operation group The difference was statistically significant(P<0.05);at 4 weeks after modeling,there was a statistically significant difference between the results of the positioning navigation test of the two model groups and the sham operation group(P<0.05),the modelⅠgroup and the sham operation Compared with the sham operation group,the difference between the escape latency period of the model group and the sham operation group was enlarged,and there was no statistically significant difference between the escape latency period of the model group 4 and the model of 1 week(P>0.05);At 1 week after the model,there was a statistically significant difference in the number of times the rats in the two model groups crossed the platform compared with the sham operation group(P<0.05),and there was no significant difference between the modelⅠgroup and the modelⅡgroup(P>0.05);At 4 weeks after modeling,there was a statistically significant difference in the number of times the modelⅡgroup traversed the platform compared with the sham operation group(P<0.05);1 week after modeling,the first traversal of the rats in the two model groups There was a statistically significant difference between the platform time and the sham operation group(P<0.05),and there was no significant difference between the modelⅠgroup and the modelⅡgroup(P>0.05);at 4 weeks after modeling,the two model groups There was a statistically significant difference between the time of the first time the rats crossed the platform and the sham operation group(P<0.05),and there was a statistically significant difference between the time of the first time the modelⅠgroup crossed the platform and the modelⅡgroup(P<0.05).The gap between the modelⅡgroup and the sham operation group in the space exploration experiment widened 4 weeks after the model was made,while the gap between the modelⅠgroup and the sham operation group narrowed.Conclusion Repeated pre-experiment can greatly reduce the mortality rate of rats.The model of bilateral common carotid artery repeated ischemia-reperfusion plus unilateral permanent ligation is more suitable for short-term research.The modeling method of blood reperfusion plus bilateral permanent ligation can be used in the long-term study of vascular dementia rats.
作者
王锦锦
赵秀秀
安素娴
宋丽娟
王月华
胡佳慧
牛梦瑶
李永
郭银珠
马慧敏
张文超
陈秀清
王维峰
WANG Jin-jin;ZHAO Xiu-xiu;AN Su-xian;SONG Li-juan;WANG Yue-hua;HU Jia-hui;NIU Meng-yao;LI Yong;Guo Yin-zhu;MA Hui-min;ZHANG Wen-chao;CHEN Xiu-qing;WANG Wei-feng(Shanxi University of Chinese Medicine,Jinzhong Shanxi 030619)
出处
《世界中西医结合杂志》
2020年第5期880-883,共4页
World Journal of Integrated Traditional and Western Medicine
基金
山西中医药大学科技创新能力培育计划项目(2018PY-035)。
关键词
血管性痴呆
大鼠
造模
Vascular Dementia
Rat
Modeling
