摘要
目的:研究降压通络方对大鼠肾小管上皮细胞凋亡的影响。方法:体外培养大鼠肾小管上皮细胞,经过缺血缺氧,将大鼠肾小管细胞随机分为4组:正常组、模型组、降压通络方组、缬沙坦组。采用CCK-8检测细胞增殖情况,免疫组化法检测大鼠肾小管上皮细胞p-AKT蛋白的表达,蛋白免疫印迹法检测大鼠肾小管上皮细胞凋亡相关基因调控蛋白Bcl-2、Bax的表达,对Bcl-2、Bax蛋白的表达水平进行相关性分析。结果:缺血缺氧呈时间依赖性抑制大鼠肾小管上皮细胞活性;免疫组化结果示:p-AKT在正常组呈高表达,模型组低表达,降压通络方组p-AKT表达明显高于模型组(P<0.01),缬沙坦组表达低于模型组(P>0.05);蛋白免疫印迹法结果示:模型组Bcl-2表达较正常组明显降低(P<0.01),降压通络方组和缬沙坦组Bcl-2的表达均高于模型组(P<0.05,P<0.01);模型组Bax表达较正常组明显升高(P<0.01),降压通络方组和缬沙坦组Bax的表达均较模型组降低(P<0.05);Bcl-2蛋白与Bax蛋白表达呈负相关性(r=-0.811,P<0.01)。结论:降压通络方可抑制大鼠肾小管上皮细胞凋亡,其作用机制可能与上调p-AKT、Bcl-2蛋白,下调Bax蛋白表达有关。
Objective: To investigate the effects of JiangyaTongluo Formula on apoptosis of renal tubular epithelial cells in rats.Methods: Rat renal tubular epithelial cells were cultured in vitro and then randomly divided into 4 groups after ischemia and hypoxia:normal group, model group, JiangyaTongluo Formula group, valsartan group. Cell proliferation was detected by CCK-8, p-AKT protein expression in rat renal tubular epithelial cells was detected by immunocytochemistry, and expression of apoptosis-related gene regulatory proteins Bcl-2 and Bax in rat renal tubular epithelial cells was detected by Western blot. Correlation analysis was conducted on the expression levels of Bcl-2 and Bax proteins. Results: Ischemia and hypoxia inhibited the activity of rat renal tubular epithelial cells in a time-dependent manner;Immunocytochemical results showed that the expression of p-AKT was high in the normal group, but low in the model group. The expression of p-AKT in JiangyaTongluo Formula group was significantly higher than that in the model group(P<0.01), and that in the valsartan group was lower than that in the model group(P>0.05);Western blot results showed that the expression of Bcl-2 in the model group was significantly lower than that in the normal group(P<0.01), while the expression of Bcl-2 in JiangyaTongluo Formula group and the valsartan group was higher than that in the model group(P<0.05, P<0.01). Bax expression in the model group was significantly higher than that in the normal group(P<0.01), while Bax expression in JiangyaTongluo Formula group and the valsartan group was significantly lower than that in the model group(P<0.05);the expression of Bcl-2 protein and Bax protein was negatively correlated(r=-0.811, P<0.01). Conclusions: The apoptosis of rat renal tubular epithelial cells can be inhibited by JiangyaTongluo Formula, and the mechanism may be related to the up-regulation of p-AKT and Bcl-2 proteins and the down-regulation of Bax protein expression.
作者
马钰
韩琳
李冰
王淑艳
沈一凡
贾祎凡
杨柳
秦建国
MA Yu;HAN Lin;LI Bing;WANG Shu-yan;SHEN Yi-fan;JIA Yi-fan;YANG Liu;QIN Jian-guo(Beijing University of Chinese Medicine,Beijing,100029,China;College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,100700,China;Tianjin Yitai Hospital,Tianjin,300011,China;Baoding The first Hospital of Traditional Chinese Medicine,Baoding,Hebei,071000,China;Dongfang Hospital,Beijing University of ChineseMedicine,Beijing,100078,China)
出处
《现代生物医学进展》
CAS
2020年第7期1211-1216,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(811734071)
北京市中医管理局北京中医药"薪火传承3+3工程"建设项目郭士魁名家研究室项目(2011-SZ-A-27)。
关键词
高血压肾损害
降压通络方
肾小管上皮细胞
缺血缺氧
细胞凋亡
Hypertensive Nephrosclerosis
Jiangya Tongluo Formula
Renal tubular epithelial cells
Ischemia-hypoxia
Cell apoptosis
