摘要
肉及肉制品营养丰富,是多种微生物良好的生长基质。本文中,笔者对肉类微生物多样性分析的主要方法进行综述,包括传统方法和分子生物学法,阐述变性梯度凝胶电泳(DGGE)、实时荧光定量PCR(RT PCR)技术和高通量测序技术(HTS)等方法的应用原理及应用实例,并对不同方法的优缺点进行比较,分析评价了高通量测序的快速、灵敏性,并对肉类微生物多样性分析方法的应用前景进行展望。
Meat and meat products are nutritious,and good substrates for microbial growth.In this paper,we review the main methods for meat microbial diversity analysis,including traditional methods and molecular biological methods.The principles and applications of denaturing gradient gel electrophoresis,real-time PCR and high-throughput sequencing are explained,the advantages and disadvantages are compared.The rapid and sensitive high-throughput sequencing method is also evaluated,and the application prospect of meat microbial diversity analysis method is prospected.
作者
甄宗圆
胡雪洁
徐留艳
王艺霖
牛玺程
ZHEN Zongyuan;HU Xuejie;XU Liuyan;WANG Yilin;NIU Xicheng(College of Food Engineering,Anhui Science and Technology University,Chuzhou 233100,China;School of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China)
出处
《生物加工过程》
CAS
2020年第3期381-385,共5页
Chinese Journal of Bioprocess Engineering
基金
安徽省重大科技专项(18030701212)
枣庄市科学技术发展计划(2019NS09)。
关键词
肉
肉制品
微生物多样性
腐败菌
高通量测序
分子生物学
食品安全
meat
meat products
microbial diversity
spoilage bacteria
high throughput sequencing
molecular biology
food safety
