摘要
目的探讨γ干扰素(IFN-γ)、骨桥蛋白(OPN)、白细胞介素-18(IL-18)在肺结核患者血清与痰液上清液中的表达与临床意义。方法收集2016年11月~2018年12月我院89例肺结核患者(肺结核组)和30例健康者(健康对照组)的血清和痰液标本,分别用酶联免疫吸附试验(ELISA)双抗体夹心法对上述3种细胞因子的水平变化进行检测,比较两组的IFN-γ、OPN、IL-18水平。结果肺结核组血清及痰液上清液中IFN-γ、OPN、IL-18的水平均高于健康对照组,差异有统计学意义(P<0.05)。结论肺结核患者血清和痰液上清液中IFN-γ、OPN、IL-18水平的升高可辅助其临床诊断,对于判断疾病转归具有重要的临床意义。
Objective To explore the expression and clinical significance of interferon-γ(IFN-γ), osteopontin(OPN and interleukin-18(IL-18) in serum and sputum supernatant from patients with pulmonary tuberculosis. Methods Serum and sputum samples were collected both from 89 patients with pulmonary tuberculosis(pulmonary tuberculosis group) and 30 healthy individuals(healthy control group) in our hospital from November 2016 to December 2018. The levels of the three cytokines mentioned above were respectively detected by double antibody sandwich enzyme linked immunosorbent assay(ELISA) technique and then the levels of IFN-γ, OPN, and IL-18 between the two groups were compared. Results The levels of IFN-γ, OPN, and IL-18 in the serum and sputum supernatant of the pulmonary tuberculosis group were higher than those of the healthy control group, and the differences were statistically significant(P<0.05). Conclusion It is of great significance for the auxiliary clinical diagnosis and the judgment of the disease prognosis due to the elevation of the IFN-γ, OPN, and IL-18 in serum and sputum supernatants from patients with pulmonary tuberculosis.
作者
杜伟勤
任建斌
DU Wei-qin;REN Jian-bin(Department of Clinical Laboratory,Lvliang People′s Hospital,Shanxi Province,Lvliang033000,China)
出处
《中国当代医药》
2020年第11期12-15,共4页
China Modern Medicine
基金
山西省吕梁市科技攻关项目(2016sfgg067-1)。
