摘要
为探明一氧化氮供体硝普钠(SNP)对铝胁迫下烟草幼苗生长和根系生理特性的促进作用,以耐铝型品种云烟100和铝敏感型品种云烟105为材料,采用水培法研究施加不同浓度SNP(0μmol/L、50μmol/L、100μmol/L、200μmol/L、400μmol/L)对铝胁迫(200μmol/L)下烟草幼苗生长、根系营养元素吸收、呼吸电子传递及内源激素含量的影响。结果表明,铝胁迫下烟草生长受到明显抑制,根系营养元素(钙、镁、铁、铜)含量、各呼吸途径的呼吸速率(总呼吸、细胞色素呼吸、交替呼吸)和根尖生长素、赤霉素含量均显著下降,而根尖活性氧(O2·-、H2O2)和脱落酸含量显著升高。加入适宜浓度的SNP能显著缓解铝对烟草根系和地上部生长的抑制,提高根系钙、镁、铁、铜营养元素含量和各呼吸途径的呼吸速率,促进根尖生长素、赤霉素和脱落酸合成,并降低根尖活性氧(O2·-、H2O2)含量。在试验浓度范围内以施加100μmol/L的SNP对烟草铝毒害缓解效果最佳,而SNP为400μmol/L时缓解效果受到抑制。综上所述,100μmol/L的SNP可通过促进烟草根系对钙、镁、铁和铜营养元素的吸收,提高根系各呼吸途径的呼吸速率以降低根尖活性氧含量,以及维持根尖内源激素的平衡而缓解烟草铝毒害。
Two tobacco cultivars of Yunyan 100(aluminum-resistant)and Yunyan 105(aluminum-sensitive)were selected to explore the effects of sodium nitroprusside(SNP,as the nitric oxide donor)with different concentrations(0μmol/L,50μmol/L,100μmol/L,200μmol/L,400μmol/L)on plant growth,root nutrient elements uptake,respiratory electron transfer and endogenous hormones contents under aluminum stress(200μmol/L)with solution culture.The results showed that aluminum stress significantly inhibited plant growth,decreased root nutrient elements(Ca,Mg,Fe,Cu)uptake,respiratory rate(total respiration,cytochrome respiration,alternative respiration),root apical auxin and gibberellin contents,but significantly increased the contents of reactive oxygen(O2·-and H2O2)and abscisic acid in root apex of tobacco seedlings.Application of SNP concentration dramatically relieved the aluminum-induced inhibition of root and aboveground growth,increased root nutrient elements(Ca,Mg,Fe,Cu)uptake,respiratory rate,the contents of auxin,gibberellin and abscisic acid in root apex,but dramatically decreased reactive oxygen(O2·-and H2O2)contents.In this experiment,SNP at 100μmol/L had the best effects on relieving aluminum toxicity in tobacco seedlings,while the alleviating effects were suppressed when SNP concentration was 400μmol/L.In conclusion,treatment with SNP at 100μmol/L could alleviate aluminum toxicity in tobacco seedlings through promoting root nutrient elements(Ca,Mg,Fe,Cu)uptake,increasing respiratory rate in order to decrease reactive oxygen(O2·-and H2O2)accumulation,maintaining balance of endogenous phytohormones in root apex.
作者
刘强
柳正葳
刘召亮
乔清华
张玉
LIU Qiang;LIU Zheng-wei;LIU Zhao-liang;QIAO Qing-hua;ZHANG Yu(College of Biology and Pharmacy,Yulin Normal University,Yulin 537000,China;College of Life Sciences,Jinggangshan University,Ji′an 343009,China)
出处
《江苏农业学报》
CSCD
北大核心
2020年第2期292-298,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31960632、31260498)。
关键词
烟草
铝胁迫
一氧化氮
营养元素
呼吸作用
植物激素
tobacco
aluminum stress
nitric oxide
nutrient elements
respiration
phytohormones
