摘要
目的 观察孕康口服液对早发性卵巢功能不全小鼠的影响.方法 采用正常动情周期ICR小鼠灌胃给予雷公藤多苷溶液40 mg/kg构建早发性卵巢功能不全模型,大鼠随机分为正常组、模型组及孕康口服液高、中、低剂量组(36、18、9 mL/kg),灌胃给药10周.给药期间定期观察小鼠动情周期;ELISA法检测血清中雌二醇(E2)、孕激素(P)、促卵泡生成素(FSH)、促黄体生成素(LH)、催乳素(PRL)、抗苗勒管激素(AMH)、抑制素B(INHB)水平;HE染色观察卵巢组织形态;TUNEL染色检测卵巢颗粒细胞凋亡;免疫组化检测Bax、Bcl-2蛋白表达.结果 与模型组比较,孕康口服液高剂量组能缩短小鼠动情周期(P<0.05),改善卵巢组织形态学并增加窦状卵泡数量(P<0.05);升高血清E2水平(P<0.05);减少卵巢颗粒细胞凋亡数量,并能降低Bax表达以及升高Bcl-2表达.结论 孕康口服液能改善雷公藤多苷致小鼠早发性卵巢功能不全,其机制可能是通过调节凋亡相关蛋白Bcl-2和Bax的表达,减少卵巢颗粒细胞凋亡.
AIM To observe the effects of Yunkang Oral Liquid(YKOL)on mice with premature ovarian in sufficiency(POI).METHODS POI models were induced by intragastric administration of tripterygium wilfordii(40 mg/kg)in ICR mice during normal estrous cycle.The mice were randomly divided into normal control group,model group and high,medium and low dose YKOL groups for 10 weeks oral administration(36,18,9 mL/kg).Periodic observation of mice in estrous cycle was made during the administration;ELISA method was used to detect estradiol(E2),progesterone(P),follicle stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),anti mullerian hormone(AMH)and inhibin B(INHB)levels;HE staining was used to observe the mor phology of ovarian tissue;TUNEL staining was used to detect the apoptosis of ovarian granulosa cells;immunohisto chemistry was used to detect Bax and Bcl 2 protein expression.RESULTS Compared with the model group,the high dose YKOL group shared shortened mice estrous cycle(P<0.05),improved morphology of ovary and in creased number of sinusoidal follicles(P<0.05);increased serum E2 level(P<0.05);reduced number of ovarian granulosa cell apoptosis,and reduced Bax expression and increased Bcl 2 expression.CONCLUSION YKOL can improve the POI induced by tripterygium polyglycoside in mice,and the mechanism may be associated with the apoptosis reduction of ovarian granulosa cells via the Bcl 2 and Bax expression regulation.
作者
许万枫
苏洁
翁路安
孙晨晨
陈素红
吕圭源
XU Wan feng;SU Jie;WENG Lu an;SUN Chen chen;CHEN Su hong;Lü Gui yuan(Zhejiang Chinese Medical University,Hangzhou 310053,China;Zhejiang University of Technology,Hangzhou 310014,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第5期1180-1186,共7页
Chinese Traditional Patent Medicine
基金
浙江省重点实验室项目(2012E10002)。
关键词
孕康口服液
早发性卵巢功能不全
卵巢颗粒细胞
凋亡
Yunkang Oral Liquid
premature ovarian insufficiency
tripterygium wilfordii
ovarian granulosa cells
