摘要
为阐明SpaA在猪丹毒丝菌致病中的作用,以临床分离的猪丹毒丝菌野生强毒株为材料采用DNA重组技术构建spaA基因敲除载体,并利用该敲除载体通过同源重组敲除强毒株的spaA基因,比较强毒株和敲除株对小鼠毒力的差异.结果表明敲除株相比野生株对小鼠的毒力降低102倍,这说明SpaA与猪丹毒丝菌的致病性有关.
In order to elucidate the pathogenic effect of SpaA,the spaA gene knockout vector was constructed from clinical isolated Erysipelothrix rhusiopahiaes wild virulent strain by DNA recombination technology.The spaA gene of the strong virulent strain was deleted by homologous recombination technology and then the virulence difference of strong virulent strains and the knockout strains was compared in mice.The result show that the virulence to mice of knockout strains decreased over 102-fold compared with wild strains.The data indicated that SpaA was related to the pathogenicity of Erysipelothrix rhusiopahiaes.
作者
陈富海
林晓君
凃彦芳
刘博婷
余璐
蔡巩林
林锦铨
彭凌
CHEN Fu-hai;LIN Xiao-jun;TU Yan-fang;LIU Bo-ting;YU Lu;CAI Gong-lin;LIN Jin-quan;PENG Ling(Henry Fok School of Biology and Agriculture,Shaoguan University,Shaoguan 512005,Guangdong,China)
出处
《韶关学院学报》
2020年第3期46-51,共6页
Journal of Shaoguan University
基金
广东省自然科学基金资助项目(2017A030307041)
广东省大学生创新创业训练计划资助项目(201810576040)
广东省科技创新战略专项资金——“攀登计划”专项资金(pdjh2020b0537).
关键词
猪丹毒丝菌
spaA基因
基因敲除
同源重组
Erysipelothrix rhusiopathiae
spaA gene
gene knockout
homologous recombination technology
