摘要
对草莓灰霉病菌具有较强拮抗活性的生防菌株F11进行分类学鉴定和抑菌效果评价,并采用正交试验和单因素试验对该菌株发酵配方和发酵条件进行优化,以期提高该菌株的发酵效率。结果表明,F11菌株对水稻稻瘟病菌、核盘菌等12种常见病原菌抑制率均达79.88%以上,表现出高效、广谱的抑菌特性,可作为植物病害生物防治的潜在资源。经菌落形态观察、生理生化测定、16S rRNA及gyrB基因测序分析,确定菌株F11为解淀粉芽孢杆菌(Bacillues amyloliqufaciens)。通过L16(4^3×2°)正交试验优化,确定该菌株适宜的发酵培养基配方为:3.00g/L牛肉膏、10.00 g/L蛋白胨、5.00 g/L NaCl、3.00 g/L葡萄糖、2.00 g/L MgCl2。单因素试验结果进一步表明,基于优化配方,菌株F11培养的适宜初始pH为7.0、温度为30℃、振荡速率为150 r/min;平板计数显示,上述培养条件下活菌数为4.67×10^9 CFU/mL。
The taxonomic identification and antimicrobial effect of a strain called F11 which has inhibitory effect on strawberry gray mold was studied.In order to improve the fermentation efficiency of the strain,the fermentation formula and conditions were optimized by orthogonal experiment and single factor test.The results showed that the strain F11 could inhibit the growth and reproduction of 12 common pathogens with high inhibition rate reaching 79.88%or more,which showed that this strain possessed broad-spectrum and highefficiency antimicrobial effect,and that it would be a hidden promising resource for biological control agent to resist plant diseases.The strain F11 was identified as Bacillus amyloliquefaciens by observation of colony morphology,physiological and biochemical tests,16S rRNA and gyrB gene sequencing.The fermentation medium of strain F11 was optimized from L16(43×26)orthogonal test:3.00 g/L beef extract,10.00 g/L peptone,5.00 g/L NaCl,3.00 g/L glucose,2.00 g/L MgCl2.Furthermore,the single factor test further showed that based on the optimized medium,the suitable initial pH of strain F11 culture was 7.0,and the befitting temperature was30℃,and the proper oscillation rate was 150 r/min,which the number of viable cells were 4.67×109 CFU/mL.
作者
陈海念
冯蓉
杨胜竹
曹本福
文明江
刘丽
陆引罡
CHEN Hainian;FENG Rong;YANG Shengzhu;CAO Benfu;WEN Mingjiang;LIU Li;LU Yingang(Agricultural College,Guizhou University,Guiyang 550025,China;Institute of New Fertilizer Resources and Technology,Guizhou University,Guiyang 550025,China;Guizhou Key Laboratory for Tobacco Quality Study,Guiyang 550025,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2020年第2期177-188,共12页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金(31760133)
贵州省科技计划(〔2017〕0106)
贵州省科技计划项目“黔科合平台人才”(〔2017〕5788号)
贵州大学人才引进项目(〔2015〕10号)
贵州省生物学一流学科建设项目(GNYL〔2017〕009)。
关键词
生防菌
鉴定
抑菌效果
正交试验
培养基优化
biocontrol bacterium
identification
inhibitory effect
orthogonal experiment
medium optimization
