摘要
目的建立同时测定白蒲黄片中黄芩素和汉黄芩素含量的方法。方法采用高效液相色谱法(HPLC)。色谱柱为Agilent C18,流动相为乙腈-水(梯度洗脱),流速为1.0 ml/min,检测波长为280 nm,柱温为30℃。结果黄芩素的回归方程为Y=24117X-223.95(r=0.9990),0.02~0.2μg黄芩素与峰面积的线性关系良好,平均回收率为98.95%,RSD为0.64%(n=9);汉黄芩素的回归方程为Y=16237X-26.698(r=0.9996),0.02~0.2μg汉黄芩素与峰面积具有良好的线性关系,平均回收率为99.28%,RSD为1.03%(n=9)。精密度、稳定性和重复性实验的RSD均<2%。结论所用方法灵敏度高、结果准确、重复性好,可用于白蒲黄片的质量控制。
Objective To establish an HPLC method for simultaneous determination of baicalein and wogonin in Baipuhuang tablets.Methods HPLC was performed on an Agilent C18 column(4.6 mm×250 mm,5 μm)using the gradient acetonitrile(A)-water(B)at the 1.0 ml/min flow rate as mobile phase. The columm temperature was 30℃ and the detection wavelength was280 nm.Results The linear regression equation of baicalein was Y=24117 X-223.95(r=0.9990);the linear range was 0.02~0.2 μg with the average recovery of 98.95% and the RSD of 0.64%(n=9). The linear regression equation of wogonin was Y=16237 X-26.698(r=0.9996);the linear range was 0.02~0.2 μg with the average recovery of 99.28% and the RSD of 1.03%(n=9). The RSD values for the precision,stability and repeatability were all less than 2%.Conclusion The established HPLC method appears to be highly sensitive,accurate and reproducible,which could be used for the quality control of Baipuhuang tablets.
作者
丁伟楠
袁新宇
刘苏
任茜
何珊
DING Wei-nan;YUAN Xin-yu;LIU Su;REN Qian;HE Shan(School of Pharmacy,Linyi University,Linyi 276000,China)
出处
《国际药学研究杂志》
CAS
北大核心
2020年第1期63-66,共4页
Journal of International Pharmaceutical Research
基金
2017年地方高校国家级大学生创新训练项目(201710452049)。
