摘要
为了调查绵羊住肉孢子虫在河南地区的感染情况,观察绵羊住肉孢子虫的形态学特点,评价住肉孢子虫对自然感染绵羊的致病特点。运用肌肉压片、石蜡切片技术和PCR方法检测绵羊住肉孢子虫感染率,观察住肉孢子虫的虫体形态及其引起的病理变化。结果显示,161份绵羊组织样品中住肉孢子虫成熟包囊肌肉压片、石蜡切片和PCR的阳性率分别为37.27%(60/161),73.91%(119/161),75.16%(121/161);其中2.52%(3/119)的样品中检测到未成熟包囊,包囊密度>50个/cm2的样品为31.93%(38/119);此外包囊周围炎、心肌细胞变性坏死、动脉血管周围炎的组织样品分别为15.97%(19/119),73.95%(88/119),8.40%(10/119)。该研究为监控绵羊住肉孢子虫病提供基础数据和参考依据。
The prevalence,morphology and pathology of Sarcocystis spp.were investigated in sheep from parts of Henan Province.The results showed that the positive rates of mature sarcocysts in sheep myocardium were 37.27%(60/161),73.91%(119/161)and 75.16%(121/161)by muscle squashed,paraffin section and PCR,respectively.Among the 119 positive samples,2.52%(3/119)immature cysts were observed,and 31.93%(38/119)of samples with 50>/cm^2 cysts were detected by paraffin section.In addition,the ratio of sarcocysts inflammation,myocardial cell degeneration and necrosis,and periarterial inflammation were 15.97%(19/119),73.95%(88/119)and 8.40%(10/119),respectively.This study provides the basic data and reference basis for the monitoring sheep sarcocystiosis.
作者
杨金柯
苏瑞景
董辉
梁虎
王林枫
杨玉荣
YANG Jin-ke;SU Rui-jing;DONG Hui;LIANG Hu;WANG Lin-feng;YANG Yu-rong(College of Animal Science and.Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2020年第4期719-723,共5页
Chinese Journal of Veterinary Science
基金
中国博士后科学基金资助项目(2016M600577)
河南省高校科技创新人才支持计划资助项目(17HASTIT038)。