摘要
了解新疆乌苏地区草原革蜱中立克次体的感染情况.采集新疆乌苏天山山地南坡的草原革蜱,通过PCR对蜱中斑点热群立克次体、贝氏柯克斯体、埃立克体、伯氏疏螺旋体、查菲埃立克体、无形体、嗜吞噬细胞无形体、牛巴贝斯虫、巴贝西原虫、利什曼原虫、恙虫立克次氏体等11种病原体进行DNA分子检测,对检出的阳性样本进行测序,并与GenBank (NCBI)中的相应基因序列进行比对分析.106只草原革蜱中斑点热群立克次体、贝氏柯克斯体、埃立克体检测结果为阳性,阳性率分别为57.23%(99/173)、37.50%(30/80)和37.74%(20/53),其余8种病原体检测结果均为阴性;对斑点热群立克次体进行序列分析,建立分子系统进化树,结果显示Guertu-Dn1801 OmpA与吉林两株M198 (MF511260.1)和M30 (MF511254.1)同源性最高,一致性均为96.90%,属劳氏立克次体;Guertu-Dn1803OmpA与俄罗斯Gorno-Altaisk-2014-H-2株(KT006594.1)和苏联246株(U43807.1)同源性最高,一致性均为94.40%,属西伯利亚立克次体;而Guertu-Dn1801 gltA和Guertu-Dn1809 gltA分别与黑龙江3LB01 (KM245567.1)和内蒙古MD0202 (KP742992.1)序列一致性均为100%,属劳氏立克次体.乌苏地区可能存在斑点热群立克次体、贝氏柯克斯体、埃立克体3种病原体的流行,且斑点热群立克次体感染率较高,应加强对当地蜱类和蜱传疾病的监测和防控.
To investigate the infection of Rickettsia in Dermacentor nuttalli of the southern slope of the Tianshan Mountain of Wusu,Xinjiang.Dermacctor nuttalli were collected in Wusu region of Xinjiang,PCR was used to detect the DNA of 11 pathogens such as spotted fever group Rickettsia,Coxiella burnetii,Ehrlichia,Borrelia,Ehrlichia chaffeensis,Anaplasma Anaplasma-phagocytophilum Babesia,Babesia spp.,Leishmani-a,Oresteia tsutsugamushi in ticks.The positive samples were sequenced and compared with the corresponding gene sequences deposited in GenBank(NCBI).Among 106 of Dermacentor nuttalli,the positive rates of infection spotted fever group Rickettsia,Coxiella burnetii and Ehrlichia were 57.23%(99/173),37.50%(30/80) and 37.74%(20/53),respectively.The other eight pathogens were all negative.The sequence analysis of spotted fever group Rickettsia was carried out and the phylogenetic tree was established.The results showed that Guertu-Dn1801 OmpA had the highest homology with Jilin province two strains M198(MF511260.1) and M30(MF511254.1) both of which were 96.9%homology,belonging to Rickettsia raoultii.Compared with Russia strain Gorno-Altaisk-2014-H-2(KT006594.1)and Soviet Union strain 246(U43807.1),Guertu-Dn1803 OmpA had 94.4% homology with them and belonged to Rickettsia sibirica.Guertu-Dn1801 gltA and Guertu-Dn1809 gltA were on the same branch with Heilongjiang province strain 3 LB01(KM245567.1) and Inner Mongolia strain MD0202(KP742992.1) respectively,and the identity was 100%,belonged to Rickettsia raoultii.There may be epidemics of spotted fever group Rickettsia,Coxiella burnetii and Ehrlich in Wusu region,and the carrying rate of spotted fever group Rickettsiae was high.Monitoring and controlling of local tick and tickborne diseases should be strengthened.
作者
孙素荣
王思远
史深
张渝疆
张敬媛
袁逸伦
杨小飞
张媛
丁焱辉
SUN Surong;WANG Siyuan;SHI Shen;ZHANG Yujiang;ZHANG Jingyuan;YUAN Yilun;YANG Xiaofei;ZHANG Yuan;DING Yanhui(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,School of Life Science and Technology,Xinjiang University,Urumqi Xinjiang 830046,China;Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi Xinjiang 830002,China)
出处
《新疆大学学报（自然科学版）》
CAS
2020年第2期190-196,共7页
Journal of Xinjiang University(Natural Science Edition)
基金
国家自然科学基金(81760365,81690369)
新疆高校科研计划自然科学重点项目(XJEDU2019I002).
关键词
草原革蜱
蜱传病原体
立克次体
遗传进化分析
Dermacentor nuttalli
tick-borne pathogens
Rickettsia
genetic evolution analysis
