摘要
使用生工柱式植物总RNA抽提纯化试剂盒、Trnzol法以及改良CTAB 3种方法,提取红肉苹果克孜阿尔玛的果肉、果皮、花瓣、叶片中的总RNA,并对比这3种方法提取RNA的产量、质量以及完整性。结果表明,由Trnzol方法提取所获得的RNA纯度较低,有降解现象,且有DNA、蛋白质的污染,果肉和果皮RNA的产量也并不理想;而用改良CTAB法和生工试剂盒法提取的RNA较少降解,所得28 S rRNA和18 S rRNA条带较为清晰,花瓣和叶片的OD260/280均在2以上,生工试剂盒法提取的RNA产量高,OD260/280和OD260/230比值均在1.8~2.5,条带的完整性好。3种方法比较而言,改良CTAB法和Tr Nzol法提取的RNA条带的清晰度、产量及质量都低于生工试剂盒法。
The total RNA extraction and purification kit, Trnzol method and improved CTAB method were used to extract the total RNA, from the pulp, pericarp, petals and leaves of red meat apple Kizi Alma, and the results of yield, quality and integrity of the three methods were compared. The final results showed that the purity of RNA extracted by T rnzol method was low, the degradation phenomenon, and the pollution of DNA, protein, the yield of pulp and pericarp was not ideal, but the RNA extracted by improved CTAB method and kit method was rarely degraded. The 28 s rRNA and 18 s rRNA bands were clear, the OD260/280 of petals and leaves was between 1.8 and 2.5, the yield of RNA extracted by raw reagent kit method was high, the ratio of OD260/280 and OD260/230 was between 2.0 and 2.5, and the integrity of the bands was good.Compared with the three methods, the clarity, yield and quality of RNA bands extracted by modified CTAB method and Tr Nzol method were lower than those by raw reagent kit method.
作者
汤蕾
童盼盼
张亚若
王芳
唐章虎
李文辉
袁祖胜
王江波
TANG Lei;TONG Pan-pan;ZHANG Ya-ruo;WANG Fang;TANG Zhang-hu;LI Wen-hui;YUAN Zu-sheng;WANG Jiang-bo(School of Plant Science,Tarim University,Alar 843300,Xinjiang,China;National Local Joint Engineering Laboratory of High efficiency and High quality cultivation and Deep processing Technology of characteristic Fruit trees in Southern Xinjiang,Alar 843300,Xinjiang,China;Xinjiang Academy of Agricultural Sciences Luntai National Fruit Tree Resource Garden,Luntai 841600,Xinjiang,China;Xinjiang production and Construction Corps fourth Division Forestry Management Station,Kedala 835099,Xinjiang,China;Agricultural Development Service Center of the 11th regiment of the first Division of the Xinjiang production and Construction regiment,Alar 843300,Xinjiang,China)
出处
《湖北农业科学》
2020年第3期144-146,153,共4页
Hubei Agricultural Sciences
基金
华中农业大学-塔里木大学科研联合基金项目(TDHNLH201601)
兵团南疆重点产业支撑计划项目(2017DB006-2)。
